WT1基因组蛋白乙酰化修饰对细胞核重编程的影响

Effect of histone acetylation modification of Wilms' tumor Ⅰ gene on nuclear reprogramming

利用体细胞移植技术获得克隆动物的成功是几十年来生命科学领域取得的重大突破之一,这项技术引起了社会的广泛关注。然而,由于哺乳动物克隆效率低下,且克隆后代发育异常等问题,已成为目前制约动物克隆技术发展和应用的瓶颈。克隆动物中经常出现后代过大综合征(LOS),该病导致克隆动物早产、难产和易夭折。LOS类似于人的伯-伟综合征(BWS),BWS也称为Wlims瘤,表现为巨舌、内脏肿大等症状。研究发现BWS的发病机理与WT1基因(Wilms’tumor 1gene)异常表达有关。本文对体细胞核重编程和表观遗传学调控细胞重编程的研究进展进行综述,并对WT1基因组蛋白乙酰化修饰与体细胞重编程之间的联系进行简要介绍,以期为生命科学领域的进一步探索与研究提供借鉴。

The birth of animals produced by somatic cell nuclear transfer（SCNT） is one of major breakthroughs in life sciences in recent decades.The SCNT technology has aroused wide concern.However,due to the low efficiency of mammalian clone and the abnormal development of cloned offspring,its development and application has been restricted greatly.Large offspring syndrome（LOS） is one of abnormal developments in the cloned animals,which leads to premature delivery,dystocia and easy to die.LOS is similar to the BO-Wei syndrome（BWS） in human.BWS is also known as Wlims＇ tumor（WT）,which performs for the giant tongue,swelling of visceral organ and other symptoms.It was found that the pathogenesis of BWS was associated with abnormal expression of WT1 gene.This review briefly describes the progress of somatic cell nuclear reprogramming and epigenetic regulation of cell reprogramming in past years.Also,it describes the relationship between the WT1 modification of histone acetylation and somatic cell reprogramming in order to provide the clue for further investigations.