蚕体和蛹粉代料培养基上的蛹虫草生长状况与品质检测

Growth and Quality Assessment of Cordyceps militaris Cultured on Bombyx mori Body and on Pupa Powder Substitute Medium

在实验室条件下以优选的蛹虫草菌株YCC-XD-2在家蚕蛹、蛾培养基和蛹粉代料培养基上培育蛹虫草,比较不同培养基上的蛹虫草的生长状况和虫草素含量,探究高产优质蛹虫草的培养方式。蛹虫草菌种在蚕体和蛹粉代料培养基上均生长良好,其中:蚕体培养基以蚕蛾培养基上的蛹虫草生长较好;蛹粉代料培养基以糯米+蛹粉培养基上的蛹虫草生长较好。蚕体培养基培育蛹虫草子实体中的虫草素含量显著高于蛹粉代料培养基培育的蛹虫草,其中,蚕蛾培养基培育蛹虫草子实体中的虫草素质量比高达21.97 mg/g。在相同培养条件下,蛹虫草子实体中的虫草素含量高于菌丝体和培养基质。试验结果表明,利用家蚕蛹、蛾培养基可以生产出高品质蛹虫草。

To delve cultivation methods for obtaining high yield and quality Cordyceps militaris,we cultured a preferentially selected Cordyceps militaris strain named YCC-XD-2 on Bombyx mori pupae and moths and on pupa powder substitute media in the laboratory and compared growth and cordycepin contents of Cordyceps militaris from different cultivation methods.It was shown that the selected Cordyceps militaris strain could grow well on both Bombyx mori body and on pupa powder substitute media.Specifically,Cordyceps militaris cultured on Bombyx mori moths grew better than that on Bombyx mori pupae,and Cordyceps militaris cultured on sticky rice plus pupa powder grew better than that on other pupa powder substitute media.The contents of cordycepin in sporocarp of Cordyceps militaris cultured on Bombyx mori bodies were significantly higher than those cultured on pupa powder substitute media.It was observed that the mass ratio of cordycepin in sporocarp of Cordyceps militaris cultured on Bombyx mori moth was as high as 21.97 mg/g.Under the same cultural condition,the content of cordycepin in sporocarp was much higher than that in mycelium and in culture medium.These results showed that high quality Cordyceps militaris can be obtained by utilizing Bombyx mori pupa and moth for the cultivation.