摘要
AIM: To determine the role of circulating tumor cells (CTCs) in prediction of the overall survival of patients with advanced malignant biliary tract obstruction. METHODS: We investigated the prognostic value of CTCs by examining two markers, cytokeratin (CK) 19 and human telomerase reverse transcriptase (hTERT) mRNA, in 40 patients diagnosed with advanced malig- nant biliary tract diseases. Quantitative real-time re- verse transcription polymerase chain reaction was used to detect CK19 and hTERT mRNA in the peripheral blood of these patients. Overall survival was analyzed using the Kaplan-Meier method and Cox regression modeling.RESULTS: Positive CK19 and hTERT mRNA expression was detected in 45% and 60%, respectively, of the 40 patients. Univariable analysis indicated that positive CK19 mRNA expression was significantly associated with worse overall survival (P = 0.009). Multivariable analysis determined that positive CK19 mRNA expres- sion, patient's age and serum bilirubin were each inde- pendently associated with overall survival. CONCLUSION: CK19 mRNA expression levels in pe- ripheral blood appear to provide a valuable marker to predict the overall survival of patients with advanced malignant biliary tract obstruction.
AIM： To determine the role of circulating tumor cells （CTCs） in prediction of the overall survival of patients with advanced malignant biliary tract obstruction. METHODS： We investigated the prognostic value of CTCs by examining two markers, cytokeratin （CK） 19 and human telomerase reverse transcriptase （hTERT） mRNA, in 40 patients diagnosed with advanced malig- nant biliary tract diseases. Quantitative real-time re- verse transcription polymerase chain reaction was used to detect CK19 and hTERT mRNA in the peripheral blood of these patients. Overall survival was analyzed using the Kaplan-Meier method and Cox regression modeling.RESULTS： Positive CK19 and hTERT mRNA expression was detected in 45% and 60%, respectively, of the 40 patients. Univariable analysis indicated that positive CK19 mRNA expression was significantly associated with worse overall survival （P = 0.009）. Multivariable analysis determined that positive CK19 mRNA expres- sion, patient＇s age and serum bilirubin were each inde- pendently associated with overall survival. CONCLUSION： CK19 mRNA expression levels in pe- ripheral blood appear to provide a valuable marker to predict the overall survival of patients with advanced malignant biliary tract obstruction.
基金
Supported by Rajavithi Hospital Project Grant and Thailand Research Fund,No.RSA52
关键词
MRNA水平
恶性肿瘤
胆道
晚期
预后
疾病
循环
mRNA表达
Circulating tumor cells
Cytokeratin 19
Human telomerase reverse transcriptase
Malignant biliary tract obstruction
Overall survival
