燕麦种子蛋白质组的GeLC-MS/MS分析

GeLC-MS/MS Analysis of Oat Seed Proteome

采用十二烷基磺酸钠-聚丙烯酰胺凝胶(SDS-PAGE)电泳结合液相色谱串联质谱方法(GeLC-MS/MS)对燕麦(Avena sativa L.)品种白燕2号种子不同溶解性的蛋白质进行分离鉴定,以期建立其种子蛋白质表达谱,为探讨干燥燕麦种子储藏及萌发的生理生化机理提供基础。结果表明:GeLC-MS/MS方法鉴定出溶解于超纯水、2.5%NaCl和70%乙醇的燕麦种子蛋白质分别为40,33和15个,共54个非冗余蛋白,其中29个蛋白至少可溶于2种溶剂。通过生物信息学软件对鉴定蛋白的分子功能、细胞组成和生物学功能进行预测,其中40个蛋白质功能得到明确的预测,涉及细胞过程、刺激应答及酶调节活性等12类。这些蛋白质与燕麦种子成熟干燥时期生命活动有关,为进一步从蛋白质水平探索燕麦种子生理代谢机理提供了理论依据。

For exploring the mechanism of biochemistry,the proteins of dry oat seeds（Avena sativa L.＇baiyan No.2＇）were extracted with MillQ water,2.5% NaCl solution and 70% ethanol then identified using SDS-PAGE and mass spectrometery approach（LC-MS/MS）.Results showed that fifty-four proteins were identified by LC-MS/MS,hereinto;twenty-nine proteins could be dissolved in two or three kinds of solutions.Forty,thirty-three and fifteen proteins could be dissolved in MillQ water,2.5% NaCl solution and 70% ethanol,respectively.The molecular function,cell components and biological pathways of fifty-four proteins were predicted by GO database.The protein functions of forty proteins were identified involving in twelve major categories including stimulate response,enzyme regulator activity,cellular processes and so on.These proteins affected the activity of dried ripe oat seed.These results provided information to explore the biochemistry mechanism of oat seeds at the protein level in the future.