摘要
采用氯仿处理、聚乙二醇沉淀和蔗糖密度梯度离心的方法,从感病的水稻叶片中分离、纯化了水稻矮缩病毒(RDV)。在琼脂糖凝胶板上电泳分离到了9个 RNA 片段的 RDV 基因组。用电泳回收的方法纯化了 RDV 基因组的各 RNA 片段。利用 ollgo dT 作为引物,合成并克隆了其中几个片段的 cDNA。通过对 cDNA 克隆的物理图谱分析,建立了亚克隆,测定了它们的 cDNA 序列。这里报告其中第8条片段的 cDNA 部分序列。
Rice dwarf virus (RDV) was isolated and purified from infected rice leaves with chloro- form extraction,PEG precipitation and sucrose gradient centrifugation.Total RDV RNA ge- home was separated in the agarose gel and segments of RDV RNA genome were purified.The cDNAs of several segments were synthesized with oligo dT as primer.Through cDNA map- ping,subcloning and sequencing,we have obtained partial DNA sequence of those segments. Here we report the cloning and partial DNA sequence of segment 8 from RDV RNA genome.
