薰衣草组培苗驯化移栽过程中形态解剖学比较

Comparative Study on Morphology and Anatomy of Lavender During Transplantation and Acclimatization

以继代培养不同时间、移栽生长不同阶段薰衣草组培苗为试材,比较研究了不同阶段组培苗叶片及根系形态解剖及超微结构差异。结果表明:瓶内壮苗培养30d左右,组培苗叶片和根系已分化出相对完整的形态组织结构,具有初步的气孔调节及光合自养能力,但与移栽50d苗和栽培1a生苗木有显著差异。温室移栽50d时,组培苗组织器官功能已基本满足露地生长的基本要求,在栽培条件合适时,可进行田间移栽相关工作。该研究为薰衣草工厂化组培苗生产的技术实施提供了科学依据。

Using subculture at different times,different stages of growth of transplanted lavender tissue culture as experimental material,anatomical structure and ultrastructure of leaves and roots were compared at different culture stages in lavender.The results showed that the relatively complete morphology tissue structure were differentiated after culturing 30 days in bottle,and had the primary capability with stoma adjustment and photoautotrophic,but there were significant differences compared with the 50 days and 1 year tissue culture seedling that grew in greenhouse and in field respectively.The function of tissue organ could basically met the requirements of field growing after growing 50 days in greenhouse,so the seedlings could be transplanted to field under the suitable conditions at this culture stage.The research provided scientific basis for the technical implementation of factory production of lavender tissue culture seedling.