毛白杨无性系30号组培再生体系的建立

Establishment of Tissue Culture Regeneration System of Populus tomentosa Clone-30

以毛白杨无性系30号的茎段为外植体,对其再生能力和再生条件进行了研究,以期建立毛白杨无性系30号的组织快繁体系。结果表明:MS培养基为比较合适的基本培养基;MS+6-BA 0.5mg/L和MS+TDZ 0.0015mg/L+NAA 0.01mg/L是其比较合适的诱导培养基,不定芽诱导率分别为86.7%和83.3%;MS+6-BA 0.7mg/L+NAA 0.05mg/L是其最佳增殖培养基,增殖率为628%;诱导毛白杨30号生根的最佳培养基为1/2MS+NAA 0.01mg/L,生根率为96.8%。

Taking stems of the P.tomentosa clone-30 as explants,the regeneration ability and conditions were studied,in order to build a rapid tissue propagation system.The results showed that the appropriate basic medium was MS and the appropriate inductive medium was MS＋6-BA 0.5 mg/L and MS＋TDZ 0.0015 mg/L＋NAA 0.01 mg/L,the shoot induction rates were respectively 86.7% and 83.3%.The optimal regeneration medium was MS＋6-BA 0.7 mg/L＋NAA 0.05 mg/L and the regeneration rate was 628%,the optimal rootage medium was 1/2MS＋NAA 0.01 mg/L,and the rate of the rooting was 96.8%.