摘要
以108杨为试材,以茎段、叶柄、叶片3种外植体的高效不定芽诱导再生为基础,研究了不同梯度浓度卡那霉素(Km)对108杨不定芽诱导的影响。结果表明:茎段外植体对Km表现出较高的耐受性,叶柄和叶片外植体的敏感性高;在遗传转化过程中70mg/L的Km作为茎段外植体的临界筛选浓度较为合适;对于叶柄和叶片外植体,选择30mg/L的Km临界筛选浓度较为合适。
Effective adventitious buds regeneration of stems,petioles and leaves of Populus×euramericana cl.‘Guariento’ were used as foundation,influence from various Kanamycin（Km） concentrations were studied.The results showed that stem was quite tolerant to kanamycin,which proper concentration was 70 mg/L in transformation;while petioles and leaves were very sensitive and an appropriate concentration was 30 mg/L.
出处
《北方园艺》
CAS
北大核心
2012年第3期119-121,共3页
Northern Horticulture
基金
林木育种国家工程实验室
林木花卉遗传育种教育部重点实验室开放基金资助项目(FOP2010-14)
国家"863"计划资助项目(2011AA100201)
2011河南科技大学大学生研究训练计划资助项目(SRTP)
关键词
108杨
离体培养
卡那霉素
Populus×euramericana cl.‘Guariento’
in vitro culture
kanamycin
