三种杀菌剂诱导彩色马蹄莲抗细菌性软腐病的研究

Study on Three Fungicides to Induce Resistance of Colour Callalily to Soft Rot

以77%的多宁可湿性粉剂、20%的叶枯唑可湿性粉剂和1.5%的噻霉酮水乳剂为试材,研究了3种杀菌剂对彩色马蹄莲抗细菌性软腐病致病菌胡萝卜软腐果胶杆菌胡萝卜亚种生长抑制效果、圆叶片法室内诱导以及活植物室外诱导彩色马蹄莲抗P.c.c.病原菌等。结果表明:3种杀菌剂对P.c.c.抑制效果不同,叶枯唑效果最好,含1 000倍～125倍的叶枯唑的PDA培养基能完全抑制P.c.c.病原菌生长;室内圆叶片法能完全抑制菌的生长,室外抑制率高于60%;多宁效果次之,室外抑制率不到50%;噻霉酮无论是对菌的生长抑制还是室内和室外诱导,都不能完全抑制;1 000倍～500倍的叶枯唑彩色马蹄莲软腐病的抑制效果差异不大(P>0.05)。

Taking 77% copper calcium sulfate,20% bismerthiazol and 1.5% benziothiazolinone as materia,the restrain effect of three fungicides was added into potato dextrose agar（PDA） medium to control the growth of P.c.c.derectly,and colour callalily was induced to resist the soft rot in the field and laboratory were studied.The results showed that P.c.c.was completely inhibited on PDA which contain 1 000 times～125 times of 20% bismerthiazol,and colour callalily could resist the growth of P.c.c.in the laboratory completely,and the inhibition rate was 60% in the field under the concentration of 1 000 times～500 times.The inhibition rate of 77% copper calcium sulfate was about 50%,which was lower than that of 20% bismerthiazol.1.5% benziothiazolinone could not inhibite the P.c.c.in the field and laboratory.The inhibition rate under the concentration of 1 000 times and 500 times of 20% bismerthiazol was not significant differences（P0.05）.