噻重氮苯基脲对贯叶金丝桃胚性愈伤诱导和分化的影响

Effects of Different Concentrations of TDZ on Embroyogenic Callus Induction and Differentiation of Hypericum perforatum L.

系统研究了不同浓度噻重氮苯基脲(TDZ)对贯叶金丝桃胚性愈伤的诱导和植株再生的影响。结果表明:诱导胚性愈伤的最适外植体为叶柄,诱导率可达100%。在诱导胚性愈伤和分化的最适培养基MS+0.05mg/L TDZ+0.2mg/L NAA上,不定芽分化率为55%。第1代培养基上,愈伤增殖迅速,绿色的胚性细胞团数目从原来的单个生长提高为平均5.6个。经2、3代培养,愈伤停止生长,胚性细胞团数量逐渐减少。

The embryogenic callus induction and shoot regeneration of Hypericumperforatum L.were studied systematically through different concentrations of TDZ.The results indicated that the most suitable part for inducing embryogenic callus was the petiole.The inducing rate reached as high as 100%.The optimized medium for inducing embryogenic callus and plant regeneration was MS＋0.05mg/L TDZ＋0.2mg/L NAA.The adventitious buds differentiating rate was 55%.The size of callus can increase greatly in the first subculture on the optimized inducing medium and the average number of green-coloured masses of embryogenic cells on each callus was 5.6.On the second and third subculture,callus stopped growing further and the number of masses of embryogenic cells decreased gradually.