土壤多环芳烃降解菌培养条件优化

Optimization of soil PAHs-Degradating bacteria culture conditions

对筛选获得的两株多环芳烃降解菌Y1和W1的培养条件进行优化,为其应用于土壤多环芳烃污染的微生物修复积累数据。采用紫外可见分光光度法测定不同温度条件下的吸光度(Absorbance)值,得出Y1和W1的最适生长温度为28℃、最适pH为7.0、最适盐浓度为2%,其中W1可在10%盐浓度下继续生长,表明W1对盐浓度的耐受性较高。分析不同浓度梯度的菲、蒽、苯并菲的溶液降解率,得出Y1对菲的最适降解浓度为25 mg/L,W1对菲的最适降解浓度为50 mg/L,Y1和W1对蒽、苯并菲的最适降解浓度为50 mg/L。

We optimized culture conditions of the two screening PAHs-Degradating bacteria Y1 and W1 to accumulate microbial remediation data in PAHs contamination of soil.We analyed the sample by using ultraviolet spectrophotometry for different temperature condition,the conclusion was that the optimum temperature for the degradation bacterium was 28℃;the optimum pH was 7.0;the optimum solt concentration was 2%.Analyzing degradation rate of the different concentration of phenanthrene,anthraquinone and triphenylene,we could concluded that the 25 mg/L was the optimum concentration for Phenanthrene by Y1,the 50 mg/L was the optimum concentration for Phenanthrene by W1,and the 50 mg/L was the optimum concentration for Anthracene and Triphenylene by Y1 and W1.