快速分析彝药桃树寄生抗氧化成分

Rapid Analysis of Antioxidant Constituents from Taxillus delavayi,a Yi Ethnomedicinal Material

目的:快速分离、鉴定彝药桃树寄生的抗氧化成分。方法:通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除实验,评价彝药桃树寄生的甲醇提取物及其二氯甲烷、正丁醇及水萃取物的抗氧化作用,利用生物活性-高效液相跟踪方法,快速分离、鉴定其有效成分。结果:从彝药桃树寄生的二氯甲烷萃取物中分离、鉴定出有效成分为松脂酚(pinoresinol)。结论:利用生物活性-高效液相跟踪方法可以快速分离、鉴定彝药桃树寄生中的抗氧化成分,可应用于其他天然植物活性成分的快速筛选。

Objectives： To rapidly separate and identify antioxidant constituents from the whole plant of Taxillus delavayi,a Yi ethnomedicinal material,and assess their antioxidant activity.Methods： The antioxidant activity of the methanol extract of the medicinal material and its different solvent fractions obtained by repeated extractions sequentially with dichloromethane,n-butanol and water was assessed by DPPH free radical scavenging assay.The dichloromethane-soluble fraction was further separated by silica gel column chromatography,and a high antioxidant component named as TDD8 was obtained and analyzed by bioassay-linked HPLC and 1H-NMR.Results： An individual compound named as TDD8-2 was separated from TDD8 by semi-preparative HPLC and identified by 1H-NMR as pinoresinol.Conclusion： The bioassay-linked HPLC method allows the rapid separation and identification of antioxidant components from medicinal materials.