摘要
目的:研究葡萄籽和皮萃取物对乳腺癌细胞迁移的抑制作用,以及对细胞活力的影响。方法:以小鼠乳腺癌细胞4T1为研究对象,四甲基偶氮唑盐(MTT)实验检测细胞增殖活性;细胞迁移实验检测细胞迁移能力;结果:质量浓度为5μg/mL的葡萄籽和皮萃取物对乳腺癌细胞的迁移具有明显的抑制作用,该抑制作用与药物质量浓度呈现剂量依赖关系;萃取物对癌细胞的活力有明显的抑制作用,葡萄籽和皮萃取物的半数有效质量浓度(IC50)分别为65.427μg/mL和72.408μg/mL。
Objective： To explore the effects of grape seed and grape skin extracts on the migration,viability and proliferation of breast cancer cells.Methods： The viability of mouse mammary carcinoma 4T1 cells was determined by MTT assay and the migration ability by transwell chemotaxis assay.Results： Both grape seed and grape skin extracts significantly reduced the migration of the 4T1 cells in a dose-dependent manner,and exhibited an obvious inhibitory effect on cell migration at a concentration as low as 5μg/mL.The extracts also obviously inhibited the viability and proliferation of the 4T1 cells with IC50（half maximal inhibitory concentration） of 65.427μg/mL and 72.408μg/mL,respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第1期239-243,共5页
Food Science
基金
上海市科委技术标准专项(07DZ05019)
关键词
葡萄籽和皮萃取物
鼠乳腺癌细胞
细胞活力
细胞迁移
grape seed and grape skin extracts
mouse mammary carcinoma cells
viability
migration
