D-半乳糖致衰老动物模型的建立及评价

Establishment and evaluation of D-galactose induced aging animal model

建立衰老动物模型是研究人类衰老过程的有效方法.结合动物的生理特性及自然属性,建立不同的动物模型,已成为衰老及抗衰老研究的焦点.D-半乳糖衰老动物模型与其他几种衰老动物模型（如自然衰老模型、臭氧损伤衰老模型、去胸腺衰老模型、SAMP系小鼠衰老模型等）相比,简便易行,价格低廉,结果稳定,因而得到广泛应用.结合该模型近年来的运用和发展,本文对该模型构建原理、剂量、具体方法和评估手段作一综述,并对行为学水平、生化水平、形态学水平、分子生物水平检测指标进行总结.总之,采用D-半乳糖120～125mg/（ kg·d）连续皮下注射6～8周是较为可靠、稳定的建立衰老动物模型的方法.

Establishment of aging animal model is an effective way to study the aging process of human being. It has been a focus to establish different aging models for research of aging and anti-aging agents according to the physiological characteristics and natural attributes of various animals. Compared with other aging models, including naturally aging model, ozone induced aging model, thymus removed aging model, and aging SAMP model, D-galactosis induced aging model has been widely used because of its easy feasibility, low lost, and stable performance. In this paper, regarding its application and development, we reviewed the D-galactosis induced aging models from the following aspects： the mechanism and the dosage of the D-galactose to induce aging, the specific protocols, and the evaluation for successful establishment. In addition, we summarized some indexes to assess aging, such as behavioral, biochemical, morphological and molecular biological indices. In summary, subcutaneous injection of 120～125mg/（kg·d） of D-galactose once per day for 6 to 8 weeks is a reliable and stable way to establish the aging model.