唾液腺腺样囊性癌中RASSF1A基因的表达

Protein expression of RASSF1A in salivary adenoid cystic carcinoma

目的:研究唾液腺腺样囊性癌(adenoid cystic carcinoma,ACC)中RASSF1A基因的蛋白表达,并分析其与ACC临床病理参数之间的关系。方法:免疫组织化学(immunohistochemistry,IHC)方法分析57例唾液腺ACC和9例瘤旁腺体中RASSF1A基因的蛋白表达,根据染色强度和染色面积对每例病例进行评分,分为0(无)、1(弱)、2(中)、3(强)4个级别。应用SPSS13.0软件包分析RASSF1A蛋白表达与患者性别、年龄、肿瘤部位、组织学分级、分期、复发、转移及患者预后之间的关系。结果:在ACC肿瘤组织的肌上皮和导管上皮的细胞质和细胞核中均见RASSF1A基因的蛋白表达。57例肿瘤组织中,21%(12/57)的病例中无RASSF1A基因的蛋白表达,47%(27/57)的病例中呈弱表达,23%(13/57)呈中度表达,9%(5/57)呈强表达。在瘤旁腺体中,RASSF1A均为强表达。统计学分析显示,RASSF1A基因的蛋白表达与肿瘤的TNM分期呈负相关(P=0.012)。RASSF1A蛋白表达低的患者,生存期显著低于表达高的患者(P=0.035)。结论:RASSF1A基因蛋白表达水平在ACC中下调,并与肿瘤分期及患者的生存期相关,提示RASSF1A基因可作为ACC患者肿瘤发展及预后判断的一个生物学标志物。

PURPOSE： To investigate the relationship between RASSF1A expression and clinicopathological features in salivary adenoid cystic carcinoma（ACC）.METHODS： RASSF1A expression was assessed by immunohistochemical analysis in 57 salivary gland ACC tissues and 9 salivary gland tissues adjacent to the tumor.According to the staining intensity and staining area,every section was scored to be 0,1,2,3.Association between RASSF1A expression and patients＇ age,sex,tumor location,histological grade,TNM stage,recurrence,metastasis and survival status were analyzed by SPSS 13.0 software package.RESULTS： RASSF1A expression was detected in cytoplasm and nuclei of ACC tumor cells,both in myoepithelial and duct cells.Among the 57 salivary gland ACC,21%（12/57） showed no RASSF1A expression（score 0）,47%（27/57） tumors were scored 1,23%（13/57） were scored 2 and 9%（5/57） were scored 3.Nevertheless,there was strong RASSF1A expression in salivary gland tissues adjacent to the tumor.Significant lower RASSF1A expression was associated with advanced clinical stage（P=0.012）.Patients with lower RASSF1A expression had a shorter survival time than those with stronger RASSF1A expression（P=0.035）.CONCLUSION： RASSF1A expression is downregulated in ACC.Low expression of RASSF1A is correlated with advanced stage and patients＇ survival in ACC,suggesting that RASSF1A may be a biological marker in progress and prognosis of ACC.