骨髓基质细胞诱导分化修复髁突软骨面缺失的实验研究

An experimental study of bone marrow stromal cells differentiating to chondrocytes for repairing temporomandibular joint condylar cartilage defects

目的:采用骨髓基质细胞(BMSCs)体内修复髁突软骨全层缺失。方法:15只山羊,9只作为实验组,将BMSCs和少量软骨细胞(7:3比例混合)按5×107/mL与生物可降解材料复合后,植入山羊髁突软骨全层缺失处;对照组6只山羊,髁突软骨全层缺失区植入支架材料,分别于术后4、8、12周每个时间段取材3只实验动物,2只对照组动物;2组分别用HE染色、Ⅱ型胶原分泌的免疫组化法进行评价。结果:实验组术后4周,山羊髁突软骨缺失区能形成成熟的软骨组织,12周时软骨未退变。对照组不能形成成熟的软骨组织。结论:骨髓基质细胞在自体软骨细胞基质的诱导下,可以修复山羊颞下颌关节髁突软骨面全层缺失。

PURPOSE： To repair the temporomandibular joint condylar cartilaginous defects by the differentiated bone marrow stroma cells combined with chondrocytes.METHODS： Fifteen goats were randomly divided into 2 groups.The experimental group included 9 goats,autologous BMSCs combined with chondrocytes were mixed with pluronic F-127 and implanted into goat temporomandibular joint condylar articular cartilage defects.The control group included 6 goats,pluronic F-127 gel was implanted into their temporomandibular joint condylar articular cartilage defects.The goats were killed 4,8,12 weeks postoperatively.The reconstructive articular cartilage was evaluated by HE staining and immunostaining of type Ⅱcollagen.RESULTS： The experimental group could reconstruct the articular cartilage after 4 weeks.12 weeks later the articular cartilage did not degenerate.The control group could not reconstruct the articular cartilage.CONCLUSIONS： BMSCs can be differentiated into chondrocytes and play an important role in repairing articular cartilage defects.