大叶杨柱头细胞ATP酶的超微结构定位

ULTRASTRUCTURAL LOCALIZATION OF ADENOSINE TRIPHOSPHATASE ACTIVITY IN STIGMA CELLS OF POPULUS LASIOCARPA AND ITS CHANGES DURING DEVELOPMENT

用细胞化学方法,对大叶杨(Populus lasiocarpa Oliv.)不同发育阶段的柱头细胞进行了 ATP 酶的超微结构定位。结果表明:(1)大叶杨柱头表膜上存在 ATP 酶的定位沉淀,但ATP 酶的活性随可授期的到来而活性增加;幼嫩期和过盛花期的柱头表膜上少或无 ATP 酶活性。显示出 ATP 酶与花粉一柱头相互作用的相关性。(2)可授期柱头细胞中,ATP 酶主要定位于线粒体内嵴、叶绿体片层、内质网、核膜及核仁、质膜和胞间连丝上,高尔基体及液泡膜上无 ATP 酶活性的定位。(3)柱头细胞在衰亡中,ATP 酶转而分布于液泡膜、消化液泡膜、线粒体外膜、叶绿体被膜及膜结构的消化残片上,而原先可授期时 ATP 酶活性最高的线粒体内嵴和叶绿体片层则失去 ATP 酶的活性。

The ultrastructural localization of adenosine triphosphatase (ATPase) activities in stigmatic cells in various developmental stages of Populus lasiocarpa was investigated using the cytoche- mical method of lead phosphate precipitation.The results show as follows: 1.Lead deposits which marked the ATPase activities were observed on the pellicle of stigmas.The ATPase activities greatly increased in receptive stage,but they were few or even absent in young and old stages.The changes of pellicle ATPase strongly exhibited that ATPase was correlated with the pollen-stigma interaction.2.In the stigma cells inreceptive stage,ATPase was mainly located at mitochondria cristae,chloroplast lamellae and endoplasmic reticulum.Lead deposits were also visible on the plasmalemma,plasmodesma,nuclear me- mbrane and in nucleoli.No lead deposits were found on dictyosome and vacuole membrane. 3.During the degeneration of stigmatic cells,the location of ATPase changed.The distribu- tion of ATPase was in vacuole membrane,digestive vesicle,mitochondrium envelop,chloroplast envelop,and digested fragment.The mitochondrium cristae and chloroplast lamellae where ATPase was the most active in previous stage now lost their ATPase activities.