摘要
大豆开花后18天,子叶细胞中的大液泡开始以分隔或缢束方式分成较小的液泡,在其中逐渐积累蛋白质,转化为蛋白体。贮藏蛋白在液泡中的积累有三种方式:一种是蛋白质逐渐沉积于液泡膜的内表面,同时伴随着液泡的出芽分离;一种是贮藏蛋白以凝集的团块结构积累于液泡中;一种是以絮状方式均匀分布于液泡内。本文对贮藏蛋白在液泡内积累的不同方式与大豆凝集素的关系,贮藏蛋白在细胞内运输的途径等问题进行了讨论。
At 18 days after flowering,it was observed that the big central vacuoles in the cotyledon cells began to subdivide or pinch-off into smaller ones,in which the storage proteins gradually accumulated,and the protein bodies thus formed.Three patterns of protein accumulation in the vacuoles were noticed,in one of them,the storage proteins deposited onto some regions of the inner surface of the tonoplast and new protein bodies formed by a budding-off process of the storage proteins enclosed by a portion of the tonoplast membrane.In other two patterns, storage proteins accumulated in vacuoles as proteinaceous lumps or as flocculent materials. The relation between the patterns of storage protein accumulation and soybean agglutinin (SBA) accumulation in vacuoles and the pathways of intracellular transport of storage pro- reins are discussed.
关键词
大豆
子叶
蛋白体发生
Soybean cotyledons
Protein body formation
Storage protein accumulation in vacuoles