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大鼠鞘内注射JWH015对瑞芬太尼诱发痛觉过敏的影响 被引量：2

Effect of intrathecal injection JWH015 on the remifentani-induced hyperalgesia in rats
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摘要 目的在大鼠切口痛模型基础上探讨大麻素受体2（cannabinoid receptor2，CB2R）激动剂JWH015对瑞芬太尼诱发的痛觉过敏的影响。方法30只雄性Sprague-Dawley（SD）大鼠采用随机数字表法分成5组（每组6只）：对照组（c组）、切口痛组（I组）、瑞芬太尼组（R组）、切口痛＋JWH015组（JI组）、切口痛＋瑞芬太尼＋JWH015组（JR组）。JI组与JR组在造模前30min鞘内注射10IxgJWH015，而C组、I组、R组均给予20％的二甲基亚砜（dimethyl sulfoxide，DMSO）溶液，容积均为10μl。除C组外，其余各组均制作切口痛模型，R组和JR组在造模的同时皮下泵注瑞芬太尼0．04mg／kg，其余组皮下泵注生理盐水，容积均为0．4ml，30min泵完。测定术前24h及术后2．6、24和48h大鼠切口手术同侧后爪的机械缩足反射阈值（paw withdrawal mechanical threshold，PWMT）和热缩足反射潜伏期（paw withdrawal thermal latency，PWTL）。结果与C组和基础值比较，I组术后各时间点PWMT和PWTL均降低（P〈0．05）；与I组比较，R组术后各时间点PWMT（6．3±0．8）、（6．4±0．8）、（6．3±1D）、（6．8±O．9）g和PWTL（12．8±1．2）、（12．2±0．9）、（13．4±1．1）、（13．5±1．3）S均明显降低（P〈0．05）；与R组相比，JR组术后6、24和48h的PWMT（7．9±1．0）、（9．9±1．1）、（8．4±1．1）g和PWTL（17．3±1．9）、（19．9±1．3）、（17．7±1．2）s明显升高（P〈0．05）。结论鞘内注射JWH-015可以有效缓解由瑞芬太尼诱发的切口周围组织痛觉过敏。 Objective To investigate the effect of cannabinoid receptor 2 （CB2R） agonist JWH015 on the hyperalgesia induced by remifentanil in a rat model of incision pain. Methods Thirty Sprague-Dawley （SD） rats were randomly divided into 5 groups （n=6）： control group（group C）, incisional pain group（group I）, incisional pain plus JWH015 group （group JI）, remifentanil group （group R）, and JWH015 plus remifentanil group （group JR）. 10 μg JWH015 diluted by 10 μl 20% dimethyl sulfoxide （DMSO）solution was intrathecally injected in each rat of group JI and JR 30 rain before plantar incision, while group C, I and R received the equivalent amount of normal saline 20% DMSO 10 μl in the same way. Plantar incision surgery was operated in group I, R, JI, and JR. In group R and JR, remifentanil （0.04 mg/kg, 0.4 ml） was infused subcutaneously with a pump for 30 min at the moment of surgical incision. The other groups were received the equivalent amount of normal saline （0.d ml） instead of remifentanil. The paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） were measurd one day before and at 2, 6, 24 and 48 h after incision. Results Compared with group C and the baseline, the level of PWMT and PWTL significantly decreased at 2, 6, 24 and 48 h after incision in group I （P〈O.05） ; Compared with group I , the significant decrease of PWMT （6.3±0.8）, （6.4±0.8）, （6.3±1.0）, （6.8±0.9） g and PWTL（12.8±1.2）, （12.2±0.9）, （ 13.4±1.1 ）, （13.5±1.3） s were observed after incision in group R （P〈0.05）. Compared with group R, the significant increase of PWMT （ 7.9± 1.0）, （9.9±1.1 ）, （ 8.4±1.1 ） g and PWTL （ 17.3±1.9 ）, （19.9±1.3）, （17.7±1.2） s were observed at 6, 24 and 48 h after incision in group JR （P〈0.05）. Conclusions Intratheeal injection of JWH015 can be effectively alleviated remifentanil-induced hyperalgesia in a rat model of incision pain.
作者 张威 刘晓杰 蒋明 刘成龙 张娟 马正良 顾小萍
机构地区 徐州医学院江苏省麻醉学重点实验室＆江苏省麻醉与镇痛应用技术重点实验室 南京大学医学院附属鼓楼医院麻醉科
出处 《国际麻醉学与复苏杂志》 CAS 2012年第2期80-83,98,共5页 International Journal of Anesthesiology and Resuscitation
基金 南京市卫生局重点课题（ZKX08028）
关键词 大麻素 大麻素受体2 JWH015 瑞芬太尼 痛觉过敏 Cannabinoids Cannabinoid receptor 2 JWH015 Remifentanil Hyperalgesia
分类号 R614 [医药卫生—麻醉学]
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参考文献19

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同被引文献36

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国际麻醉学与复苏杂志
2012年 第2期

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