摘要
关于线粒体的发育的研究,较多地以动物和酵母为对象,植物较少。而线粒体发育过程中 H^+-ATP 酶的 F_1-ATP 酶变化的研究,目前尚未见报道。本文以豌豆子叶为材料,就线粒体的发育,H^+-ATP 酶活性变化以及这种变化与 F_1-ATP 酶亚基组成的相关性进行了初步研究,其最终目的是探讨线粒体发育过程中内膜的发生及膜蛋白的组装问题。
Electron microscopic observation indicated that the mitochondrial membrane of pea co- tyledon gradually developed into integral structure during seeds imbibition.ATP-synthesizing activity of H^+-ATPase increased in company with mitochondrial development,but the content of F_1-ATPase subunits was not different on the mitochondria of cotyledon imbibed for 6 hours and for 24 hours in water.After cotyledon was imbibed at low temperature,the content of γ and β subunits of F_1-ATPase was distinctly reduced with the inhibition of H^+-ATPase activity.
关键词
豌豆
子叶
线粒体
发育
酶
Pea
Cotyledon
Mitochondria
Development
H^+-ATPase
F_1-ATPase
