田菁胶生物合成中的甘露糖磷酸变位酶

PHOSPHOMANNOMUTASE IN THE BIOSYNTHESIS OF SESBANIA GUM

用 DE-52和磷酸纤维素亲和层析分离和纯化了甘露糖磷酸变位酶(PMM),非 SDS 凝胶电泳为一条带,但 SDS 凝胶电泳则有三条带,分子量为41500,66000和74000。测定了田菁(Sesbania cannabina)种子的子叶和胚乳中的甘露糖磷酸变位酶和葡萄糖磷酸变位酶(PGM)的活性,并进行了比较,讨论了两种酶的耐温性与田菁胶合成生理活动的相关性。

The phosphomannomutase (PMM) was isolated and purified by DE-52 and phosphocel- lulose column chromatographies.Non-SDS and SDS gel electrophoresis showed,respectively, one and three bands with molecular weights of 41500,66000 and 74000.The maximal activity of PMM at 30℃ was examined,and the comparison of the results with those of phosphoglu- comutase (PGM) were made due to close relation between the two enzymes.In addition,the relationship between the characteristics of the enzymes and the physiology in Sesbania gum synthesis was discussed.