RP-HPLC定量三乙胺法测定中药黄连中盐酸小檗碱的含量

Content determination of Berberine hydrochloride in coptis chinensis franch by RP-HPLC with the quantification of Triethylamine

目的:建立一种反向高效液相色谱(RP-HPLC)法测定黄连中盐酸小檗碱的含量。方法:采用HPLC法,色谱柱为Luna C18柱(150 mm×4.60 mm,5μm),流动相为乙腈-0.1%磷酸(25∶75),每1 000 ml0.1%的磷酸溶液中加入50μl的三乙胺(扫尾剂)进行洗脱;检测波长为340 nm。结果:采用HPLC法检测黄连中盐酸小檗碱的含量,其在0.246～2.460μg范围内与峰面积积分值呈良好的线性关系。平均加样回收率为100.1%,相对标准偏差(RSD)为1.60%。结论:HPLC简便可行、重复性好,可作为检测盐酸小檗碱含量的质量控制;一定量的三乙胺分析纯可作为很好的扫尾剂,用于盐酸小檗碱的含量测定。

Objective： To establish a new RP-high-performance liquid chromatography（HPLC） method for detecting the content of Berberine hydrochloride（BH） in coptis chinensis franch.Methods： The content of BH in coptis chinensis franch was deteminated by HPLC.HPLC conditions for the column： Luna C18（150 mm×4.60 mm,5 μm）,acetonitrile-0.1% phosphoric acid（25 ∶75）,and adding 50 μl Triethylamine（tailing-suppressing reagent） into every 1 000 ml 0.1% phosphoric acid for elution,the detection wavelength was 340 nm.Results： This HPLC method exhibited a linear relationship between the content of BH within 0.246 to 2.460 μg and the peak area integration,the average recovery rate of BH was 100.1%,the relative standard deviation（RSD） was 1.60%.Conclusion： This is a simple,reproducible and reliable HPLC method for determinating the content of BH.A certain amount of Triethylamine analytically pure reagent is a very good tailing-suppressing reagent for using HPLC method to determinate the content of BH.