伽马刀放疗腹膜后淋巴结转移癌对肠道菌群的影响

The impact of γ knife radiotherapy for retroperitoneal lymph node metastases on intestinal microflora

目的:观察腹膜后淋巴结转移癌γ刀放疗后肠道菌群的变化。方法:选取经临床表现及影像学、病理学确诊的腹膜后淋巴结转移癌并拟行γ刀放疗患者40例为观察组,健康志愿者20例为正常对照组,采用实时荧光定量聚合酶链式反应(PCR)检测观察组及正常对照组粪便中大肠杆菌、肠球菌属、双歧杆菌属及乳酸杆菌属细菌数量,并对两组结果进行比较分析。结果:与正常对照组比较,观察组患者γ刀放疗前及放疗后1周时粪便中4种目标菌群细菌数量无明显变化(P>0.05);γ刀放疗1个月后,粪便中大肠杆菌及肠球菌属细菌数量仍无显著变化(P>0.05),但双歧杆菌属及乳酸杆菌属细菌数量明显减少(P<0.05)。结论:使用γ刀放疗腹膜后淋巴结转移癌1个月不会引起大肠杆菌及肠球菌属细菌数量的改变,但可导致双歧杆菌属及乳酸杆菌属细菌数量明显下降。

Objective： To observe the change of intestinal microflora after γ knife radiotherapy in patients with retroperitoneal lymph node metastases.Methods： In this test,40 patients with retroperitoneal lymph node metastases were selected as observation group,they were diagnosised by clinical manifestations,imaging and pathologic findings,all these cases were given γ knife radiotherapy.Tweenty healthy volunteers were selected as the control group.The number of E.coli,Enterococcus,Bifidobacterium and Lactobacillus in early morning stool of these patients were detected before γ knife radiotherapy,1 week after radiotherapy,1 month after radiotherapy,and the healthy control group by real-time fluorescence quantitative PCR,and every target bacteria was analysized.Results： Compared with the control group,the 4 kinds of target bacteria in the facels of the patients before γ knife radiotherapy and 1 week after radiotherapy had no significant change（P0.05）.One month after radiotherapy,the number of E.coli and Enterococcus still had no significant change（P0.05）,but the number of Bifidobacterium and Lactobacillus decreased significantly（P0.05）.Conclusions： Using γ knife radiotherapy to therapy retroperitoneal lymph node metastases couldn′t cause the number of E.coli and Enterococcus changes in a month,but it could cause the number of intestinal Bifidobacterium and Lactobacillus decrease significantly.