摘要
目的:观察腹膜后淋巴结转移癌γ刀放疗后肠道菌群的变化。方法:选取经临床表现及影像学、病理学确诊的腹膜后淋巴结转移癌并拟行γ刀放疗患者40例为观察组,健康志愿者20例为正常对照组,采用实时荧光定量聚合酶链式反应(PCR)检测观察组及正常对照组粪便中大肠杆菌、肠球菌属、双歧杆菌属及乳酸杆菌属细菌数量,并对两组结果进行比较分析。结果:与正常对照组比较,观察组患者γ刀放疗前及放疗后1周时粪便中4种目标菌群细菌数量无明显变化(P>0.05);γ刀放疗1个月后,粪便中大肠杆菌及肠球菌属细菌数量仍无显著变化(P>0.05),但双歧杆菌属及乳酸杆菌属细菌数量明显减少(P<0.05)。结论:使用γ刀放疗腹膜后淋巴结转移癌1个月不会引起大肠杆菌及肠球菌属细菌数量的改变,但可导致双歧杆菌属及乳酸杆菌属细菌数量明显下降。
Objective: To observe the change of intestinal microflora after γ knife radiotherapy in patients with retroperitoneal lymph node metastases.Methods: In this test,40 patients with retroperitoneal lymph node metastases were selected as observation group,they were diagnosised by clinical manifestations,imaging and pathologic findings,all these cases were given γ knife radiotherapy.Tweenty healthy volunteers were selected as the control group.The number of E.coli,Enterococcus,Bifidobacterium and Lactobacillus in early morning stool of these patients were detected before γ knife radiotherapy,1 week after radiotherapy,1 month after radiotherapy,and the healthy control group by real-time fluorescence quantitative PCR,and every target bacteria was analysized.Results: Compared with the control group,the 4 kinds of target bacteria in the facels of the patients before γ knife radiotherapy and 1 week after radiotherapy had no significant change(P0.05).One month after radiotherapy,the number of E.coli and Enterococcus still had no significant change(P0.05),but the number of Bifidobacterium and Lactobacillus decreased significantly(P0.05).Conclusions: Using γ knife radiotherapy to therapy retroperitoneal lymph node metastases couldn′t cause the number of E.coli and Enterococcus changes in a month,but it could cause the number of intestinal Bifidobacterium and Lactobacillus decrease significantly.
出处
《东南大学学报(医学版)》
CAS
2012年第1期72-78,共7页
Journal of Southeast University(Medical Science Edition)
关键词
腹膜后淋巴结
转移癌
γ刀放疗
肠道菌群
实时荧光定量聚合酶链式反应
retroperitoneal lymph node
metastases
γ knife radiotherapy
intestinal microflora
real-time fluorescence quantitative polymerase chain reation