照山白醇提物对大鼠缺血心肌的保护作用

Protective Effect of Ethanol Extracts from Rhododendron micranthum on Myocardial Ischemia in Rats

目的:研究照山白醇提物对异丙肾上腺素(IS0)致大鼠心肌缺血模型的作用。方法:采用大鼠皮下注射IS0造成心肌缺血模型,抽取暴露腹腔大鼠的腹主动脉血,取得其血清进行检测。采用试剂盒方法,测定血清中肌酸激酶(CK)、肌酸激酶MB同工酶(CK-MB)和乳酸脱氢酶(LDH)的活性,及血清中钙、镁、钾、钠的浓度。结果:照山白醇提物可有效抑制心肌缺血大鼠模型血清CK(P<0.001)、CK-MB(P<0.001)和LDH(P<0.001)活性的升高,及血清中钙(P<0.001)、镁(P<0.05)、钾(P<0.05)、钠(P<0.001)等离子浓度的改变,均具有统计学意义。结论:照山白醇提物可以改变大鼠缺血心肌的理化参数,具有较明显的保护作用。

Objective： To study the pharmacological effects of the ethanol extracts from Rhododendron micranthum on myocardial ischemia induced by isoprenaline （ISO） in rats. Method： The rat myocardial ischemia model was set up by ISO subcutaneous injec- tion. The serum samples were obtained through exposed abdominal aorta and detected by kit method. The activity of CK, CK-MB and LDH and the contents of Ca2 ＋ , Mg2 ＋ , Na ＋ and K ＋ in the serum samples were determined. Result： The ethanol extracts from Rhododen- dron micranthum could significantly restrain the activity increase of CK （P 〈 0. 001 ）, CK-MB （P 〈 0. 001 ） and LDH （P 〈 0. 001 ） and the content changes of Ca2 ＋ （ P 〈 0. 001 ） , Mg2 ＋ （ P 〈 0.05 ） , K ＋ （ P 〈 0. 05 ） and Na ＋ （ P 〈 0. 001 ） in the serum of myocardial ische- mia rats caused by ISO. Conclusion： The ethanol extracts of Rhododendron micranthum can change the chemical and physical parame- ters of myocardial ischemia rats and show protective effects on myocardihl ischemia rats caused by ISO.