摘要
使用电击法和 PEG 法将 CAT 和 GUS 基因分别与 CaMV 35S 启动子构成的两种嵌合基因,转移到水稻、玉米、烟草、胡萝卜和香石竹(Dianthus caryophyllus)等品种的原生质体中,均得到了瞬间表达。在电击法中,大多数转化后玉米、烟草和胡萝卜原生质体表现出明显的基因活性。转化效率与所用仪器的电击参数有关。PEG 转化中 PEG 处理时间较国外报道稍有缩短,但转化后的水稻、烟草、胡萝卜和香石竹样品中均得到了阳性结果。
Two chimeric genes—CAT and GUS genes under the control of CaMV 35S promoter were transferred into the protoplasts isolated from cultivars of rice,corn,tobacco,carrot and carnation grown in China by electroporation and PEG pathway.Transient expression of these genes were obtained in all transformants.With electroporation,most of transformed corn,to- bacco and carrot protoplasts showed CAT or GUS activity and the effeciency was related to parameters of the electroporater.In PEG pathway,the time of PEG incubation was shorter than those reported in most papers and positive results were obtained.
