盐酸二甲胺四环素纳米复合微球凝胶的制备及其抑菌作用研究

Preparation of minocycline hydrochloride nano-composite microsphere gel and its antibacterial characteristics in vitro

目的:制备盐酸二甲胺四环素纳米复合微球凝胶并检测其体外抑菌性。方法:制备盐酸二甲胺四环素-聚乳酸乙醇酸共聚物-脂质体复合物(minocycline hydrochloride-poly-lactic-co-glycolic acid-Li-posome,MH-PLGA-Liposome),选用卡波姆(carbomer,CP)作为赋形剂,制备粘度合适的MH-PLGA-Liposome纳米复合微球凝胶。采用打孔法比较MH-PLGA-Liposome纳米复合微球凝胶与派丽奥对牙周主要致病菌牙龈卟啉单胞菌(Porphyromanus gingivalis,Pg)、具核梭杆菌(Fusobacterium nucleatum,Fn)和变异链球菌(Streptococcus mutans,Sm)的抑菌效果。结果:以合适配比的卡波姆∶水(71.3∶2)作为赋形剂制备了粘度合适的MH-PLGA-Liposome纳米复合微球凝胶,其对牙周主要致病菌(Pg、Fn)和Sm均有一定的抑菌作用,与派丽奥相比两者抑菌效果无统计学差异(P>0.05)。结论:以聚乳酸乙醇酸共聚物-脂质体核壳(PLGA-Liposome)为载体并以卡波姆作为赋形剂制备的盐酸二甲胺四环素纳米复合缓释微球凝胶具有良好的抑菌效果。

AIM：To preparate minocycline hydrochloride nano-composite microspheres gel and to test its antimicrobial characteristics in vitro.METHODS：Minocycline hydrochloride nano-composite microspheres（MH-PLGA/Liposome） were prepared,Carbopol was used as a excipient to prepare MH-PLGA/Liposome nanocomposite microsphere gel.The antibacterial effects of the gel on main periodontal pathogens（P.gingivalis,F.nucleatum） and caries pathogen S.mutans.were determined by drilling method and compared with Periocline.RESULTS： Carbopol at appropriate ratio（71.3：2） was chosen to prepare MH-PLGA/Liposome nano-composite microsphere gel with appropriate viscosity.The antibacterial effects of MH-PLGA/Liposome nanocomposite microsphere gel on P.gingivalis,F.nucleatum and S.mutans were similar to that of Periocline（P0.05）,which showed good antimicrobial activity.CONCLUSION：The MH-PLGA/Liposomes nano-composite microsphere gel has good antibacterial effect,and its physical properties are suitable for clinical usage.