宫颈鳞状上皮癌变过程中水通道蛋白3的表达研究

Expression and Significance of Aquaporin 3(AQP 3) in Cervical Carcinogenesis

目的:揭示水通道蛋白3(AQP3)在维吾尔族妇女宫颈癌发生、浸润过程中的表达及意义。方法:应用RT-PCR、Westernblot和免疫荧光染色检测AQP3在82例宫颈癌、36例宫颈上皮内瘤样病变Ⅲ级和42例正常宫颈中的表达。结果:在正常宫颈和宫颈鳞癌组织中AQP3 mRNA和蛋白均表达,蛋白水平表达有差异(P<0.05),而mRNA表达无差异(P>0.05)。AQP3在正常宫颈、宫颈上皮内瘤样病变Ⅲ级和宫颈癌中的阳性率分别为19.05%、27.78%和42.68%,阳性表达率存在显著性差异(P<0.05)。结合临床病理资料分析,AQP3阳性表达率与宫颈癌患者临床分期、肿瘤直径、浸润深度呈正相关(P<0.05),与宫颈癌大体分型、病理分级和淋巴结转移无关(P>0.05)。结论:AQP3在子宫颈鳞癌疾病发生、肿瘤生长和局部浸润过程中可能发挥重要的生理作用。

Objective： To reveal the expression and the clinical significance of aquaporin 3 （ AQP 3 ） in cervical carcinoma tis- sues from Uygur patients. Methods： The expression of AQP3 in 10 cases with chronic cervical inflammation and 27 cases of cervical carcinoma was determined using real-time PCR, Western blot analysis, and immunofluorescence. The expression of AQP3 protein in 82 cases with cervical carcinoma, 36 cases with Stage-III cervical intraepithelial neoplasia （ CINⅢ ）, and 42 cases with chronic cervical in- flammation, was assayed using immunohistochemistry. Results： AQP3 mRNA and protein were expressed in both the chronic cervical inflammation and cervical carcinoma groups. The AQP3 protein expression was statistically significant （ P 〈 0.05 ）, whereas the AQP3 rnRNA expression was not （ P 〉 0.05 ）. Moreover, the positive rate of AQP3 was 19.00%, 27.78%, and 41.46% in the chronic cervical inflammation, CIN Ⅲ, and cervical carcinoma groups, respectively, with significant differences among the positive expression （ P 〈 0.05 ）. The expression of AQP3 in cervical carcinoma had no correlation with histological grade, histological type, or lymph node me- tastasis （ P 〉 0.05 ）, however, it had significant association with FIGO stage, diameter of tumor, and invasive depth （ P 〈 0.05 ）. Con- clusion： The expression ofAOP3 nlavs an irnnortant role in the carcinogenesis and progression of cervical carcinoma.