fMRI观察静息态下健康人睡眠剥夺前后脑局部一致性差异

Resting-state fMRI observation on difference of regional homogeneity in healthy subjects after normal sleep and sleep deprivation

目的运用fMRI和局部一致性(ReHo)方法观察受试者睡眠剥夺(SD)前后静息态脑神经功能的变化。方法对15名健康受试者分别在正常睡眠情况下(正常态组)和24hSD状态下(SD组)间隔2周行静息态fMRI扫描。采用ReHo计算、分析数据,以SPM5软件进行配对t检验分析,设定差异有统计学意义(P<0.001)的体素体积(V)为≥270mm3。结果与正常态组比较,SD组ReHo值升高的脑区主要包括左侧额中回,右侧中央前回、颞叶横回,双侧中央后回;ReHo值降低脑区包括左侧额中回、右侧额下回及右侧小脑后叶。结论 SD引起多个脑区形成的网络发生功能紊乱,其中以处于情绪调控环路中的相关脑区变化明显。静息态fMRI可以反映受试者SD状态下脑部功能区ReHo的改变。

Objective To explore the the difference of abnormal brain activity in healthy subjects after normal sleep and after sleep deprivation（SD） with resting-state fMRI and regional homogeneity（ReHo） method.Methods Totally 15 healthy subjects underwent resting-state fMRI scans after normal sleep（normal situation group） and after 24hSD（SD group）,and the scans were repeated after 2weeks.ReHo method was used to calculate and analysis the data.SPM5software was used to perform two-pair t test.P0.001and cluster volume more than or equal to 270mm 3 were regarded as statistical significance.Results Compared with normal situation group,SD group showed higher ReHo in left middle frontal gyrus,right precentral gyrus and right transverse temporal gyrus,bilateral postcentral gyrus,while lower ReHo in left middle frontal gyrus,right inferior frontal gyrus and right cerebellum posterior lobe.Conclusion SD caused abnormal changes in whole network changes by connection of multiple brain regions,especially the brain regions which are in emotional control loop.Resting-state fMRI can reflect the changes of ReHo in functional regions of SD subjects.