余甘子对大鼠酒精性脂肪肝的炎症抑制作用研究

Ameliorative effect of Phyllanthus emblica on inflammatory response in rat alcoholic fatty liver disease model

目的观察余甘子对大鼠酒精性脂肪肝中炎症反应的抑制作用。方法通过高脂及乙醇混合饲料喂养大鼠8周以诱导酒精性脂肪肝的形成,同时每日给予一部分大鼠余甘子萃取物0.06 mL/g以研究余甘子的保护作用。8周后检测各组大鼠血生化和肝脏中相关炎症指标。结果相较于对照组,酒精肝大鼠血清中谷丙氨酸转氨酶(ALT)和天门冬氨酸转氨酶(AST)显著上升(P<0.01),肝组织切片中可见明显炎症位点及脂肪颗粒累积,肝脏中炎症因子(TNF-α,IL-1β和COX-2)的表达量亦显著上升(P<0.01)。加入余甘子协同处理的大鼠以上指标均有明显改善(P<0.05或P<0.01)。结论在大鼠酒精肝模型中余甘子对酒精肝有治疗作用。

Objective To investigate the ameliorative effect of Phyllanthus emblica on inflammatory response in rat alcoholic fatty liver disease （AFLD） model. Methods Rats were fed high-fat and ethanol diet for 8 weeks to induce AFLD. During the development of AFLD, some of the rats were injected with 0.06 mL/g extracts of Phyllanthus emblica per day. After 8 weeks, serum and hepatic samples were collected for measurements. Results AFLD rats showed significantly higher levels of ALT and AST in serum （P 〈 0.01）. Fat accumulation and inflammatory sites were also seen in AFLD rat livers, accompanied by up-regulation of hepatic pro-inflammatory mediators （P 〈 0.01）. Co-treatment of Phyllanthus emblica effectively ameliorated AFLD phenotypes （P 〈 0.05 or P 〈 0.01）. Conclusion Phyllanthus emblica plays protective roles in rat AFLD model.