早期血清pNF-H和S-100B的含量变化对颅脑损伤患者疗效的影响

The early change and influencing factor of effect in treatment of serum pNF-H and S-100B in patients with traumatic brain injury

目的探讨颅脑损伤患者(早期)血清中神经丝蛋白H磷酸化亚型(pNF-H)和S-100B水平的变化对其疗效的影响。方法采用酶联免疫吸附法(ELISA)检测40名健康人和118例颅脑损伤患者损伤后6h、12h、24h、3d、5d、7d的血pNF-H和S-100B水平,分析pNF-H与格拉斯哥昏迷评分及预后的关系。结果观察组各时间点检测血pNF-H和S-100B浓度均显著高于对照组,差异有统计学意义(P均<0.05);观察组内中、重型亚组各时间点检测血pNF-H浓度均显著高于轻型颅脑损伤亚组,差异有统计学意义(P均<0.05);重型亚组各时间点血清pNF-H浓度均显著高于中型颅脑损伤亚组,差异有统计学意义(P均<0.05);颅脑损伤后24h的血pNF-H水平与24h的GCS及6个月后格拉斯哥预后评分(GOS)呈负相关(r=-0.47、-0.33,P均<0.05)。结论血pNF-H和S-100B水平检测可作为判断颅脑损伤早期病情和疗效的指标之一。

Objective To explore the early changes and curative effect of serum pNF-H and S-100B level in patients with traumatic brain injury. Methods Serum pNF-H and S-IOOB level of 40 healthy persons and 118 patients with traumatic brain injury were detected by ELISA method, and analysis the relationship with GCS and prognosis. Results The pNF-H and S-100B level of patients were higher than that of the control group （P 〈 0.05）; the pNF-H level of medium and heavy group were higher than that of light group （P 〈 0.05）; the pNF-H level of heavy group was higher than that of medi- umgroup（P 〈 0.05）; the pNF-H level of 24h after TBI has negative correlation with 24h GCS（P 〈 0.05） and 6month GOS（P 〈 0.05）. Conclusion pNF-H and S-100B level examinations can be used for diagnosis of early brain injury and predict prognosis.