复方中药“天芪航力片”提取物对+Gz致大鼠脑损伤的防护作用

Protective effects of compound Tianqi tablet extraction on +Gz-induced brain injuries in rats

目的:观察复方中药"天芪航力片"提取物对+10 Gz暴露后大鼠脑损伤的影响,进一步探讨其可能的防护作用机制。方法:36只雄性SD大鼠随机分为对照组、+10 Gz组和"天芪航力片"防护组,每组12只。于灌胃给药14 d后,给予+10 Gz、5 min的加速度暴露,观察各组大鼠脑超微结构、脑ATP酶含量、血清超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及血液流变学的变化。结果:脑组织电镜和组织化学染色结果显示,与对照组比,+10 Gz组大鼠脑皮层神经细胞出现线粒体肿胀、嵴模糊不清及溶酶体增多等损伤变化,大鼠脑ATP酶含量显著降低(P<0.05);"天芪航力片"防护组脑组织损伤较轻,大鼠脑ATP酶含量无明显变化。与对照组和+10 Gz组比,"天芪航力片"防护组SOD均显著升高(P<0.05)。与对照组相比,+10 Gz组的高、中、低切全血黏度,血浆黏度均显著升高(P<0.05),"天芪航力片"防护组,高、中、低切全血黏度、红细胞压积均显著下降(P<0.05);与+10 Gz组相比,"天芪航力片"防护组,高、中、低切全血黏度、红细胞压积均显著下降(P<0.05)。结论:复方中药"天芪航力片"提取物对+10 Gz暴露引起的脑损伤具有保护作用,其防护机制可能与改善高G暴露引起的大鼠血液流变学改变、降低脂质过氧化损伤及减轻能量代谢障碍有关。

Objective： To observe the effects of Fufang Tianqi tablet extraction on rats brain injuries after ＋10 positive acceleration（Gz） exposure and further to investigate the potential mechanisms of the protection.Methods： Totally 36 male SD rats were randomly divided into control group,＋10 Gz group and Tianqi tablet groups.Forteen days after drug administration,the rats were exposed to ＋10 Gz for 5 min.Changes of rats brain ultrastructural parameters,brain ATP,blood serum superoxide dismutase（SOD）,malondialdehyde（MDA） and hemorheological were detected after ＋10 Gz exposure.Results： The brain electron microscope and histochemical staining results displayed the injury changes in rats brain such as brain cell appeared mitochondrial swelling,cristae unclear,lysosomes increased and the content of ATP enzymes was significantly lower（P〈0.05）in ＋10 Gz group than that in control group;the rats brain injuries changes was slight and the content of ATP enzymes positive cell has not obviously changed in Tianqi tablet group than that in control group.Compared with control group and ＋10 Gz group,the SOD was significantly higher（P〈0.01） in Tianqi tablet group.Compared with control group,the high,medium and low shear blood viscosity and blood plasma viscosity were obviously higher（P〈0.05） in ＋10 Gz group,while the high,medium and low shear blood viscosity and the hematocrit were significantly lower（P〈0.05） in Tianqi tablet group;the high,medium and low shear blood viscosity and the hematocrit were significantly lower in Tianqi tablet group than that in ＋10 Gz group（P〈0.05）.Conclusion： Fufang Tianqi tablet extraction can protect rats against brain injuries after ＋10 Gz exposure,the potential mechanism of brain injuries protection may be the Fufang Tianqi tablet extraction can improve the ＋Gz-induced hemorheology change,lipid peroxidation damage and lessen the energy metabolism dysbolismus.