环氧合酶-2介导促炎因子脂多糖诱导的脊髓长时程增强的变化

COX-2 mediates changes of LPS-induced spinal long-term potentiation

目的探讨环氧合酶-2(COX-2)和促炎因子脂多糖(LPS)对脊髓长时程增强(LTP)的作用。方法采用细胞外记录技术,在脊髓腰膨大部记录背角浅层神经元C-纤维诱发电位,蛋白质印迹检测LPS处理后不同时间点COX-2蛋白的表达。结果腹腔注射LPS后4 h脊髓LTP显著增强,COX-2蛋白的表达显著增高;LPS注射后12、24 h对脊髓LTP无明显作用,COX-2的表达无明显增强;COX-2的选择性抑制剂NS398可翻转LPS注射4 h后对脊髓背角LTP的增强效应,NS398可显著抑制LPS 4 h后对脊髓COX-2表达的增强。结论 COX-2的激活参与LPS诱导的脊髓LTP的变化。

Objective To explore the roles of cycclooxygenase-2（COX-2） and pro-inflammatory cytokine lipopolysaccharide（LPS） in spinal long-term potentiation（LTP）.Methods C-fiber-evoked field potentials were recorded at superficial layers of the spinal dorsal horn at the lumbar enlargement,and COX-2 expression in LPS-treated rats at different time points was measured by immunoblotting technique.Results Spinal LTP and COX-2 expression were significantly augmented 4 h,but not 12 h and 24 h after intraperitoneal injection of LPS.NS398,a selective inhibitor of COX-2,reversed the elevated LTP induced by LPS 4 h after injection.Elevated COX-2 expression in LPS-treated rats 4h after injection was significantly suppressed by NS398.Conclusion Activation of COX-2 is involved in changes of LPS-induced spinal LTP.