期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

^(131)I-rMIF荷瘤小鼠体内生物学分布及肿瘤靶向性研究 被引量：2

Bio-distribution and tumor targeting of ^(131)I-rMIF in tumor-bearing mice
原文传递
导出
摘要 目的研究放射性碘131标记基因重组巨噬细胞移动抑制因子(recombinant macrophage migration inhibitionfactor,rMIF)在荷瘤小鼠体内分布及肿瘤靶向性,为肿瘤早期诊断提供新的制剂。方法利用Iodogen(四氯二苯基甘脲)法放射性碘131标记rMIF(131I-rMIF),体外研究肝癌细胞系H22对该制剂的摄取;小鼠尾静脉注射131I-rMIF,研究其在H22荷瘤小鼠的生物学分布特征及放射自显影特征。结果成功制备了131I-rMIF,生物学活性良好。标记率87.34%,放化纯94.95%。室温放置48 h仍保持稳定。在0.5、1、2和4 h时,肝癌细胞H22对131I-rMIF的摄取率均明显高于对Na131I的摄取率(P<0.05)。生物学分布研究表明,小鼠尾静脉注射131I-rMIF后,肝、肾的放射性较高,其他脏器放射性较低。荷瘤模型在注射后1、3、6、24 h肿瘤/对侧肌肉(T/NT)的放射性比值分别为2.701±0.230、3.931±0.281、4.242±0.111和3.587±0.241。荷瘤鼠的放射自显影显像显示131I-rMIF注射后6 h后可以在肿瘤部位明显浓聚。结论131I-rMIF标记物稳定,标记率高,在荷肝癌小鼠中肿瘤靶向性分布明显,值得进一步研究。 Objective To evaluate bio-distribution and tumor targeting of 131I-labeled recombinant macrophage migration inhibition factor（rMIF） in tumor-bearing mice,and to provide an imaging marker for early diagnosis of hepatocellular carcinoma.Methods MIF was labeled with 131I using the Iodogen method and the labeled rate and stability were identified with paper chromatography.Specific binding with hepatocellular carcinoma H22cells in vitro was analyzed by cells uptake assay.Bio-distribution and tumor targeting were analyzed after injection of 131I-rMIF through the tail vein in tumor-bearing mice.Results 131I-rMIF,with good bioactivity,was successfully prepared.The labeled rate of 131I-rMIF was 87.34%,radiochemical purity was 94.95%,and the stability was good.The uptake of 131I-rMIF by tumor cells was much higher than that of the controls（free Na131I） at 0.5,1,2 and 4 h（P0.05）.131I-rMIF was mainly metabolized through the liver and kidney.And higher radioactivity was detected within tumors（target）.T/NT（target-to-non-target） ratios were 2.701±0.230,3.931±0.281,4.242±0.111 and 3.587±0.241 at 1,3,6 and 24 h after injection,respectively（P0.05）.The result of autoradiography showed that 131I-rMIF was specifically localized in tumors after 6 h.Conclusion 131I-rMIF,with good stability,good labeled ratios and rapid targeted distribution in tumor cells,and may be used for detection of hepatocellular carcinoma.
作者 姜士芹 张超 梁婷 宋静 孙虎魁 侯桂华
机构地区 山东大学医学院实验核医学研究所
出处 《山东大学学报（医学版）》 CAS 北大核心 2012年第1期81-84,88,共5页 Journal of Shandong University:Health Sciences
关键词 巨噬细胞移动抑制因子 放射性核素 生物学分布 肝肿瘤 小鼠 Macrophage migration inhibition factor Radioisotope Biodistribution Liver neoplasms Mice
分类号 R817.3 [医药卫生—影像医学与核医学]
  • 相关文献

参考文献13

  • 1Seeff L B,Hoofnagle J H.Epidemiology of hepatocellu-lar carcinoma in areas of low hepatitis B and hepatitis C endemicity[J].Oncogene,2006,25(27):3771-3777.
  • 2Robert A.Mechanisms and effectors of MIF-dependent promotion of tumourigenesis[J].Cell Signal,2004,16(1):13-19.
  • 3Eiji H,Takashi O,Pipok K D,et al.Overexpresion of macrophage migration inhibition factor induces angiogene-sis and deteriorates prognosis after radical resection for hep-atocellular carcinoma[J].Cancer,2005,103(3):588-596.
  • 4Zhang C,Liang T,Song J,et al.Evaluation of macro-phage migration inhibitory factor as an imaging marker for hepatocellular carcinoma in murine models[J].Scandina-vian Journal of Gastroenterology,2011,46(6):720-726.
  • 5ZhaoY M,Wang L,Dai Z,et al.Validity of plasma macrophage migration inhibitory factor for diagnosis and prognosis of hepatocellular carcinoma[J].IntJ Cancer,2011,129(10):2463-2472.
  • 6Lamki L M,Zukiwski A A,Shanken L J,et al.Radio-imaging of melanoma using Tc-labeled fab fragment reac-tive w ith a high molecular w eight melanoma antigen[J].Cancer Research,1990,50(3):904-908.
  • 7侯桂华,于敏,李璐娜.小鼠MIF在大肠杆菌中的表达及纯化[J].山东大学学报（医学版）,2003,41(5):469-471. 被引量：5
  • 8Bloom B R,Bennett B.Mechanism of areactionin vitro associated w ith delayed-type hypersensitivity[J].Sci-ence,1966,153(731):80-82.
  • 9H.Conroy,L.Mawhinney,S.C.Donnelly.Inflamma-tion and cancer:macrophage migration inhibitory factor(MIF)—the potential missing link[J].Q J Med,2010,103(11):831-836.
  • 10Agarwal R,Whang D H.Maerophage migration inhibi-tory factor expression in ovarian cancer[J].Am J Obstet Gyneeol,2007,196(4):348.

二级参考文献7

  • 1萨姆布鲁克主编.分子克隆实验指南[M].北京：科学出版社,1992..
  • 2Bemhagen J, Calandra T, Mitchell RA, et al. MIF is a pituitary-derived cytokine that potentiales lethal endotoxemia[J]. Nature, 1993, 365:756.
  • 3Bemhagen J, Bather M, Calandra T, et al. An essential role for macrophage migration inhibitory factor in the tuberculin delayed type hypersensitivity reaction[J].J Exp Mcd, 1996, 183:277.
  • 4de lone YP, Abadia-Molina AC, Satoskar. Development of chronic colitis is dependent on the eytokine MIF[J].Nature Immunology, 2001, 2(11):1061.
  • 5Brown FG, Nikole Paterson DI,Chadban SI. Urine MIF concentration as a diagnotie tool in human renal allograft rejection[J]. Transplantation, 2001,71(12):1777.
  • 6Kamimura A, Kamachi M. Intracellular distribution of macrophage migration inhibitory factor predicts the prognosis of patient with adenocarcinoma of the lung[J]. Cancer, 2000, 89(2):334.
  • 7Kitaichi-N, Ogasawara-K. Different influence of macrophage migration inhibitory factor in signal transduction pathway of various T cell subsets [J]. Immunobiology,2000, 201(3-4):356.

共引文献4

同被引文献23

  • 1Rhee S H, Im E, Pothoulakis C. Toll-like receptor 5 en- gagement modulates tumor development and growth in a mouse xenograft model of human colon cancer[ J ]. Gas- troenterology ,2008,135 (2) : 518-528.
  • 2Xu J, Lowey J, Wiktund F, et al. The interaction o f four genes in the inflammation pathway significantly pre- dicts prostate cancer risk[ J]. Cancer Epidemiol Biomark- ers Prey ,2005,14 ( 11 ) :2563-2568.
  • 3Zhou M, McFarland-Mancini M M, Funk H M, et al. Toll-like receptor expression in normal ovary and ovarian tumors[ J ]. Cancer Immunol Immunother, 2009,58 ( 9 ) : 1375-1385.
  • 4Cai Z, Sanchez A, Shi Z, et al. Activation of toll-like re- ceptor 5 on breast cancer cells by flagellin suppresses cell proliferation and tumor growth [ J ]. Cancer Res, 2011,71 ( 7 ) :2466-2475.
  • 5Park J H, Yoon H E, Kim D J, et al. Toll-like receptor 5 activation promotes migration and invasion of salivary gland adenocarcinoma[J ]. J Oral Pathol Med, 2011,40 (2) :187-193.
  • 6Rydberg C, Mfmsson A, Uddman R, et al. Toll-like re- ceptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas [ J ]. Immunology, 2008,128 ( 1 Suppl) : 600-611.
  • 7Jacobson O, Weiss I D, Szajek L P, et al. PET imaging of CXCR4 using copper-64 labeled peptide antagonist[ J]. Theranostics, 2011, 1 : 251-262.
  • 8Weber M S, Starck M, Wagenpfeil S, et al. Multiple sclerosis: glatiramer acetate inhibits monocyte reactivity in vitro and in vivo [ J ]. Brain,2004,127 ( Pt 6) : 1370-1378.
  • 9Liu S, Hsieh W Y, Jiang Y, et al. Evaluation of a 99mTc-labeled cyclic RGD tetramer for noninvasive ima- ging integrin (xV ~33-positive breast Cancer[ J]. Bioconju- gate Chem,2007,18(2) :438-446.
  • 10Yoshida Y, Kurokawa T, Sawamura Y, et al. The posi- tron emission tomography with F-18 17 beta-estradiol has the potential to benefit diagnosis and treatment of endom- etfial cancer [ J ]. Gynecol Oncol, 2007, 104 ( 3 ) : 764- 766.

引证文献2

山东大学学报（医学版）
2012年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部