三叶青多糖抗肝损伤作用的研究

Anti-liver Damage Activity Analysis of Polysaccharide in Radix Tetrastigmatis Hemsleyani

目的测定三叶青中多糖的含量,并研究三叶青多糖抗四氯化碳(CCl4)致急性肝损伤的作用。方法采用硫酸-苯酚法测定三叶青多糖的含量,然后将ICR小鼠随机分为5组,即空白组、模型对照组、阳性对照组,三叶青多糖高、低剂量组,分别灌胃等容积的生理盐水、联苯双酯和三叶青多糖,给药一段时间后给小鼠腹腔注射四氯化碳致其肝损伤,检测其血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、丙二醛(MDA)及超氧化物歧化酶(SOD)水平。结果三叶青中多糖的含量为8.69%;与模型组比较,三叶青多糖治疗组ALT、AST及MDA含量均明显降低(P<0.01),而SOD水平显著提高(P<0.01)。结论三叶青多糖对CCl4造成的小鼠急性肝损伤具有阻抗作用。

To determine the content of polysaccharide in radix tetrastigmatis hemsleyani,then to observe the effects of polysaccharide in radix tetrastigmatis hemsleyan on acute liver injury in mice. Methods The content of polysaccharide in radix tetrastig- matis hemsleyani was determined by phenol - sulfuric acid method. Then 40 mice of ICR species into were randomly divided 5 groups： blank control group, model group, positive control group, lowdose group and highdose group. These mice were given physiological saline, bifendate and polysaccharide in radix tetrastigmatis hemsleyani by intragastric administration every day. After a week, carbon tetrachloride was injected to cause liver injury in mice. Then serum alanine aminotransferase （ALT） ,aspartate aminotransferase （AST） , malondialde- hyde（MDA） and uperoxide dismutase（SOD） levels was determined. Results The content of polysaceharide in radix tetrastigmatis hems- leyani was 8.69%. Compared with model group, ALT, AST and MDA levels were significantly reduced（ P 〈 0.01 ） ,while SOD levels were significantly increased in treatment group of polysaecharide（P 〈 0.01 ）. Conclusion Polysaccharide in radix tetrastigmatis hemsleyani has good impedance function on CC14 - induced liver injury in mice.