大黄素对腹部术后大鼠ghrelin表达的影响

Effect of Emodin on Ghrelin Expression from Rats after Abdominal Surgery

目的探讨大黄素对腹部术后大鼠生长激素释放肽(ghrelin)的影响及其可能机制。方法 126只SD大鼠,120只随机分为大黄素高中低组、假手术组、模型对照组5组,另6只备用,造模3、6、12、24h后分别测定血清生长抑素(SS)、生长激素释放肽、胃底组织ghrelin表达水平、胃内残留率、肠推进率及肠管炎症损伤程度。结果①各组死亡率均为0;②高剂量(24、12、6h)组,中剂量6h组血清SS水平明显低于对照组(P<0.05);血清ghrelin水平高剂量(24、12h)组、低剂量24h组明显高于对照组(P<0.05);胃底组织ghrelin表达水平高剂量3h、中剂量24h、低剂量3h组明显高于对照组(P<0.05);高剂量12h组、中剂量(24、6h)组、低剂量(24、6h)组小肠推进率明显高于对照组(P<0.05);高剂量组24h,低剂量3h组胃内残留率明显低于对照组(P<0.05);炎症评分高剂量组(3、6、24h)低于对照组(P<0.05);胃底组织ghrelin表达,高剂量组3h明显高于中剂量组(P<0.05);③中剂量组12h血清SS水平及小肠推进率明显高于高剂量组;胃内残留率高剂量组12h明显高于中剂量组(P<0.05)。结论大黄素能双向调节腹部术后大鼠胃肠动力,可能与减轻肠管炎症反应、抑制生长抑素、提前和提高ghrelin分泌峰值有关。

To approach the mechanism of the action of emodin on the ghrelin secretion from rats after abdominal surger- y. Methods Totally 120 SD rats after abdominal operation were randomly divided into five groups：as sham -operated, model control, emodin with low dosage（ EL）, medium dosage（EM） and high dosage （EH） groups. Each group was divided into four subgroups randomly, and rats were treated with emodin after postoperation. The gastric remainder rates of Blue Dextron （BD） 2000, intestinal emptying rate, level of serum ghrelin, the pathological changes in intestinal canal, somatostatin（ss） and expression of ghrelin from gastric fundus were measured post - operatively on 3,6,12,24h after administration with fasted. Results ①The mortality of rats in all groups were 0. ②The contents of serum ss （at 6, 12 and 24h in EH groups, at 12 and 24h in EM groups） , the gastric remainder rates of BD 2000 （at 24h in EH groups, at 3h in EL groups） were significantly lower than those in the model control group （P 〈0.05）. The contents of serum ghrelin （at 12, 2gh in HE groups, at24h in EL groups）, the expression of ghrelin from gastric fundus （at 3h in EH groups, at 24h in EM groups, at 3h in EL groups）, the intestinal emptying rate （at 12h in EH groups, at 6, 12h in EM groups, at 6, 24h in EL groups） were significantly higher than those in the model control group （ P 〈 0.05 ）. The pathological severity scores in intestinal canal in the Sham - op- erated groups, at 5h in EH groups, at 24h in EM groups and at 3h in EL groups were also significantly lower than those in the model con- trol group （P 〈 0.05 ）. The contents of serum ghrelin at 12h and the expression of ghrelin from gastric fundus at 3, 6h in the Sham - oper- ated groups were significantly higher than those in the model control group （ P 〈 0.05 ）. （~）The expression of ghrelin from gastric fundus at 3h in EH groups were significantly higher than those in the EM group （ P 〈 0.05 ）. The expression of ghrelin from gastric fundus （ at 24h in EM groups, at 12h in EH and EM groups）, the contents of serum ss （at 12h in EH groups, at 6h in EM groups）, the gastric remain- der rates of BD 2000 （ at 12h in EH groups） , the intestinal emptying rate （ at 12h in HE groups） were significantly lower than those in the model control group （ P 〈 0.05 ）. Conclusion The study showed that emodin had dyadic adjust function to gastrointestinalmotility by alte- ring the level of serum SS and secretory peak of ghrelin.