血浆内毒素定量检测的临床应用价值探讨及参考值范围的调查

Clinical Value of Plasma Endotoxin Quantity Determination and Investigation on its Reference Range for Normal Values

目的探讨血浆内毒素定量检测的临床应用价值,并建立温州健康人群血浆内毒素浓度的参考值范围。方法采用动态浊度法定量检测242例不明原因发热(fever of unknown origin,FUO)患者和252例健康受检者血浆内毒素浓度,用SPSS13.0软件对其结果进行统计学分析,并采用95%可信区间确定其参考值范围。结果发热组血浆内毒素浓度明显高于健康组,其中位数分别为8.76pg/ml与2.07pg/ml,差别有统计学意义(P<0.05)。健康受检者男组血浆内毒素浓度虽低于女组,但差别无统计学意义(P>0.05);不同年龄段的血浆内毒素浓度虽均有所不同,但差别亦无统计学意义(P>0.05);以95%可信区间取值,确定健康人群血浆内毒素浓度的参考值范围为0～4.14pg/ml。结论血浆内毒素浓度的定量检测,可以对FUO患者是否存在内毒素血症做出早期判断,从而有助于其病因的快速确定,给临床治疗提供一定的帮助;厂商提供的血浆内毒素浓度参考值范围不适合本实验室,建议不同实验室建立各自的参考值范围。

To discuss the clinical value of plasma endotoxin quantity determination, and to establish the reference range for normal values of plasma endotoxin in Wenzhou areas. Methods Plasma endotoxin of 242 patients with fever of unknown origin （ FUO ） and 252 healthy people were detected by turbidimetric. The results were analyzed by SPSS 13.0 software. And 95% confidence interval was used to determine normal reference values. Results The level of plasma endotoxin in FUO group was higher than that of healthy group （ P 〈 0.05 ）. The result of female group was higher than that of male group, and the level of plasma endotoxin altered slightly among age groups. However ,there were no statistical significances（ P 〉 0. 05 ）. The normal value of plasma endotoxin was 0 -4.14pg/ml. Con- clusion Detecting endotoxin in plasma will help to determine endotoxemia early and it can provide definite help for clinic diagnosis of FUO. The reference range for normal values of plasma endotoxin provided by manufacturers wasn＇t suit for our laboratory,and it suggested that the reference range for normal values of plasma endotoxin be established in different laboratories respectively.