组织蛋白酶D对口腔鳞癌中VEGF-C和VEGF-D的影响及淋巴管生成作用的研究

Clinical Study of Lymphangiogenesis of Cath-D in Oral Squamous Cell Carcinoma

目的检测组织蛋白酶D(Cath-D)对口腔鳞癌中血管内皮生长因子-C(VEGF-C)和血管内皮生长因子-D(VEGF-D)表达的影响,探讨其对淋巴管生成的作用。方法选择86例口腔鳞癌标本作为观察组,收集60例正常口腔黏膜组织作为对照组,应用流式细胞分析Cath-D、VEGF-C和VEGF-D蛋白在两组中的表达,关注其表达的相关性及临床价值。结果口腔鳞癌中Cath-D、VEGF-C和VEGF-D高表达,口腔鳞癌中Cath-D、VEGF-C和VEGF-D的表达与肿瘤的分化程度、炎细胞浸润及淋巴结转移的差别有统计学意义。口腔鳞癌中Cath-D与VEGF-C、Cath-D与VEGF-D均呈正相关。结论口腔鳞癌中Cath-D、VEGF-C和VEGF-D表达明显增加,Cath-D能促进VEGF-C和VEGF-D的表达,进而促进淋巴管的生成。联合检测Cath-D、VEGF-C和VEGF-D的表达可能对判断肿瘤的预后有参考价值。

To detect the expressions of Cath - D, VEGF - C and VEGF - D in oral squamous cell carcinoma, study their clinical significance and relationship, for directing clinical work. Methods The observation group included 86 cases of oral squa-mous cell cell carcinoma, and the control group included 60 cases of normal oral mucosa. The expressions of Cath - D, VEGF - C and VEGF- D were detected in two groups by flow cytometry. We analyzed their clinical significance and relationship in the observation group. Results The expressions of Cath - D, VEGF - C and VEGF - D were obviously higher in the observation group than those in the control group. The expressions of Cath - D, VEGF - C and VEGF - D were obviously different in differention, inflammation and lymph node metastasis in the observation group. There was positive correlationship between Cath - D and VEGF - C, Cath - D and VEGF - D in oral squamous cell carcinoma. Conclusion The higher expressions of Cath - D, VEGF - C and VEGF - D may promote the development of oral squamous cell carcinoma. Cath - D can promote the pathway of VEGF - C／VEGF - D, induce the lymphangiogenesis in neoplasm. The expressions of Cath - D, VEGF - C and VEGF - D may be significant prognostic indicators in oral squamous cell carcinoma.