摘要
用水稻叶绿体Ca^(2+)-ATP酶研究了Al^(3+)的植物毒性与钙调素(CaM)的关系。外源CaM可激活此酶,证明叶绿体Ca^(2+)-ATP酶是受CaM调节的。CaM对此酶的激活作用可以被CaM拮抗剂TFP和CPZ所抑制。Al^(3+)对CaM激活作用的影响类似于CaM拮抗剂、Ca^(2+)的加入可缓解Al^(3+)的抑制作用。认为Al^(3+)毒害作用的一种可能模式为:Al^(3+)→CaM→靶酶→代谢活动。
The relationship between aluminium phytotoxicity and calmodulin has been studied with calcium-dependent ATPase in chloroplasts of rice. This enzyme could be activated by extrin- sic calmodulin. It showed that the activity of Ca^(2+)--ATPase in chloroplasts was regulated by calmodulin. The activation of calmodulin to the enzyme might be inhibited by calmodulin an- tagonists, TFP and CPZ. The effects of Al^(3+) on the activation of calmodulin was similar to that of the calmodulin antagonists. Calcium could reduce the inhibition of aluminium. It seems that there is a model of toxic responses in plants to aluminium: Al^(3+)→calmodulin→target enzy mes→metabolism.
