室内条件下云斑天牛成虫相遇行为反应

Behavioral responses of encountering adults of the white-striped longhorn beetle,Batocera lineolata(Coleoptera:Cerambycidae),in the laboratory conditions

为了探索云斑天牛Batocera lineolata Chevrolat雌雄成虫的交配机制,采用室内饲养观察和视频轨迹捕捉系统(Etho Vision3.1)自动记录分析相结合的方法,对云斑天牛的两性相遇行为进行了研究。行为仪分析结果表明,雌雌、雄雄与雌雄相遇过程中,雌雄在轨迹相交时间和净相对运动上显著长于雌雌和雄雄(P<0.05),而在反应前时间上显著短于雌雌和雄雄相遇情况(P<0.05)。室内试验观察表明,云斑天牛成虫相遇包括避让、打斗和交配3种行为。雌雌相遇发生避让的频率最高为80.98%(P<0.05)。雄雄相遇发生避让的频率为78.03%,显著高于发生打斗的频率21.96%(P<0.05);雌、雄成虫与正在交配的一对成虫相遇发生避让的频率显著高于另外3种行为[打斗(继续交配)、打斗(结束交配)、打斗(与后来者交配)](P<0.05),雌、雄成虫发生避让、打斗(继续交配)和打斗(结束交配)3种行为的发生频率存在性别差异(P<0.05);当雄雄相遇中成虫是初次相遇时,在发生打斗的频率上显著高于再次相遇(P<0.05),成虫在雄雄相遇发生避让的频率上,再次相遇显著高于初次相遇(P<0.05),雌雄成虫初次相遇发生交配的频率显著高于再次相遇的频率(P<0.05)。云斑天牛成虫相遇行为的研究为研究云斑天牛召唤机制、性信息素的生物合成及成虫繁殖行为学提供了依据。

In order to search the mating system of male and female adults of the white-striped longhorn beetle,Batocera lineolata Chevrolat,we studied the encountering behavior of male and female of B.lineolata by laboratory observation in combination with automatic record and analysis through video tracking capture system（EthoVision 3.1）.The test results by behavior instrument showed that the intersection time of two tracks and net relative movements between one male and one female were longer than that between one female and one female or between one male and one male during the encountering process（P〈0.05）,while their reaction time was shorter than that for female-female or male-male（P〈0.05）.In the laboratory experiment,it was observed that the encountering process of B.lineolata included three behaviors,i.e.,avoidance,fight and mating.During female-female encountering process,the maximum avoidance rate was 80.98%（P〈0.05）.In male-male encountering,the avoidance rate was 78.03%,which was much higher than the fight rate of 21.96%（P〈0.05）.Avoidance rate between one male or female and a couple under mating process was much higher than that for another three behaviors,i.e.,fight（continue to mate）,fight（finish mating） and fight（mate with new comers）（P〈0.05）.There was a sexual difference（P〈0.05） in occurrence frequency of these three bahaviors,i.e.,avoidance,fight（continue to mate） and fight（finish mating） between the male and the female.If it was the first encountering for two males,the fight rate was much higher than that for their second encountering（P〈0.05）,and the avoidance rate for their second encountering was much higher than that for the first time（P〈0.05）.But the mating rate for the first encountering of a male and a female was much higher than that for their second encountering（P〈0.05）.The study upon encountering behavior of B.lineolata provides the basis for studying calling mechanism and sex pheromone biosynthesis of B.lineolata as well as reproductive behavior of the adults.