基于均匀设计与支持向量回归的棉铃虫幼虫全纯人工饲料配方优化

Optimization of chemically defined diet for larvae of the cotton bollworm(Helicoverpa armigera) based on uniform design and support vector regression

昆虫全纯人工饲料配方是精细研究昆虫营养生理的前提。本研究以棉铃虫Helicoverpa armigera幼虫为供试对象,以本研究室前期发展的多因素多水平配方优化实验设计与分析方法UD-SVR为指导,以略加修改的苹浅褐卷蛾Epiphyas postvittana幼虫全纯人工饲料为初始配方,先优化20种天然氨基酸,再优化蔗糖等其他8种非氨基酸营养组分,经4轮97个处理组合,获得一个棉铃虫幼虫全纯人工饲料满意配方,平均蛹重、化蛹率分别由初始配方的0.160g和10.4%高效增至满意配方的0.255g和97.9%。该全纯人工饲料满意配方的获得,不但再次证实了UD-SVR方法的有效性,同时为进一步深入研究棉铃虫营养需求与营养代谢奠定了基础。

In order to get a satisfying formula of the chemically artificial diet for larvae of the cotton bollworm,Helicoverpa armigera,we used UD-SVR,an experimental design and analysis method reported by our preliminary studies,for formula optimization.Based on the benchmark diet of a chemically artificial diet for the larvae of the light brown apple moth,Epiphyas postvittana,the contents of 20 natural amino acids were optimized first,and the contents of 8 non-amino acid nutrients including sucrose were optimized then.After 4 rounds of optimization with total 97 schemes,an optimal formula of the chemically artificial diet for the cotton bollworm larvae was obtained.When the H.armigera larvae fed on this diet,their mean pupal weight and the pupation rate were 0.255 g and 97.9%,respectively;when the larvae fed on the benchmark diet,however,the above two indices were only 0.160 g and 10.4%,respectively.The results reconfirmed the effectiveness of UD-SVR in the complex prescription optimization.More importantly,the optimized chemically artificial diet for the cotton bollworm provides the basis for further research on the requirement and metabolism of nutrition compounds in the moth.