摘要
本研究利用叶盘(Leaf disc)法将细菌 CAT 基因(氯霉素乙酰转移酶基因)转入番茄栽培品种462的细胞中,通过组织培养,获得了能在含卡那霉素培养基上生长的转化体再生植株.经 DNA 分子杂交及蛋白质检测,证明 CAT 基因已插入植物基因组中,并已在植物组织中表达。
Bacterial CAT gene with the 35S promoter of CaMV was transferred into leaf discs of cultivar Lycopersicon esculenturn 462 with the help of Ti plasmid of Agrobacterium tume- jaciens.These leaf discs were placed on MS salt and B5 vitamine medium containing kana- mycin and carbenicillin.Several kinds of phytohormones were chosen in the medium,and it was found that zeatin and NAA have great effects on shooting and rooting.Transgenic to- mato plants showed their normal flowering and fruiting. Leaves of these transgenic tomato plants were used for assaying the gene expression.Re- sults of Southern Blot showed that the CAT gene was stably integrated into the genome of transgenic plants.The bacterial CAT proteins were also detected in transgenic tomato leaves with immunoreaction of CAT antibody.The methods presented here for culturing transformed tomato cells will be a great help for transfering economically important genes into cultivars of tomato plants in China.
