翠云草总黄酮的提取工艺研究

Study on the Extraction Technology of Total Flavonoids from Selaginella uncinata

目的:优化翠云草总黄酮的提取工艺,提高翠云草总黄酮的提取率。方法:通过单因素试验,采用紫外分光光度法,以总黄酮得率为指标,分别考察乙醇浓度、提取时间、提取次数和溶剂用量对提取效率的影响,并在此基础上设计L9(34)正交试验,研究翠云草乙醇回流提取法的提取工艺。结果:翠云草总黄酮乙醇回流提取法的最佳工艺条件为:用料液比1∶35的60%乙醇,加热回流3次,每次1.5 h。结论:采用正交设计法对翠云草中总黄酮提取条件优化合理可行,为提高翠云草中总黄酮的提取率提供了实验基础,并为其进一步的工业化生产提供科学的理论依据。

Objective：To optimize the extraction technology of total flavonoids and improve the extraction ratio of total flavonoids from Selaginella uncinata.Methods： Using the content of flavonoids as the index determined by UV spectrophotometry,chose single factor experiment to investigate the total flavonoids content influenced by four factor ethanol concentration,extracting time,extraction times and solvent content on the influence of extraction efficiency.And on the basis,L9（34） orthogonal experiment was carried out.Results： The ethanol reflux extraction conditions were as tollows：extracted in 60% ethanol（solid-liquid rate at 1∶ 35） for 3 times and heated for 1.5 hours per time.Conclusion： The optimum extraction by the orthogonal design method is rational and feasible,provides experimental basis for improving the content of the total flavonoids and further scientific theoretical basis for industrial production of the total flavonoids from Selaginella uncinata.