二次回归正交设计法优选黄芩苷醇提工艺

Quadratic Regression-orthogonal Design Optimization Ethanol Extraction Craft of Baicalin

目的:采用二次回归正交试验设计法优化黄芩苷醇提工艺参数。方法:以黄芩苷转移率为考察指标,采用高阶统计的方法对试验设计参数进行优选,并预测分析。结果:最优提取工艺为:乙醇浓度64%,提取时间115min,乙醇用量11.6倍,此时黄芩苷转移率达到85.21%。结论:二次回归正交试验设计法用于中药有效成分的提取,具有应用简便、预测性好、精确度高等优点。

Objective： To optimize the extraction process of baicalin using the quadratic regression-orthogonal design.Methods： The influential factors were ethanol doses,ethanol concentration and extract time.Used the high level statistical method to design experiment parameter and forecast analysis results.Results：The optimization conditions of extraction was 64%ethanol,115 min for extract time,volume of use 11.6 times and it′s extraction rate was 85.21%.Conclusion：It shows that the optimum model is simple,highly precision and highly predictive.