银杏多糖的分离鉴定和体外抗氧化活性测试

The Purification and Characterization of Polysaccharides Isolated from Ginkgo biloba and Their in vitro Antioxidant Activities

目的:对银杏多糖进行分离纯化,单糖组成确定并对其抗氧化活性进行测试。方法:用热提醇沉的方法提取多糖,使用Sevag法除蛋白,粗多糖用DEAE-52和DEAE-Sepharose Fast Flow进行色谱分离,精多糖用凝胶排阻色谱法测定相对分子量(Mr),红外光谱检测糖构型,高效离子色谱法测单糖组成,在体外测定其对羟基自由基、DPPH.的消除作用。结果:两个精多糖GBPB-W和GBPB-S的分子量分别为26 300和19 100,均由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、甘露糖组成,比例分别为(3.48∶8.47∶3.73∶1.76∶1)和(5.34∶5.37∶5.27∶1∶1.68)。GBPB-S具有一定的清除自由基的能力。结论:银杏多糖GBPB-S在体外有直接的抗氧化活性。

Objective：To isolate and identify the polysaccharides of Ginkgo biloba and determine their antioxidant activities in vitro.Methods： Used hot extraction and alcohol precipitation to get the crude polysaccharides,removed protein with Sevag method,further purify by column chromatography packed with DEAE-52 and DEAE-Sepharose Fast Flow.The homogeneity and molecular weights were evaluated,configuration and monosaccharide composition were measured by IR and high performance ion chromatography analysis,the scavenging activities of GBPB-S on hydroxyl radical and DPPH were measured.Results： The molecular weights of GBPB-W and GBPB-S were 26 300 and 19 100,and both of them were composed of rhamnose,arabinose,galactose,glucose and mannose with the ratios of（3.48∶ 8.47∶ 3.73∶ 1.76∶ 1） and（5.34∶ 5.37∶ 5.27∶ 1∶ 1.68）.GBPB-S had certain scavenging effect on hydroxyl radical and DPPH.Conclusion： The polysaccharide isolated from Ginkgo biloba has a direct antioxidant activities in vitro.