摘要
本文以玉米秸秆活性氧固体碱蒸煮浆(木素脱除率82.1%)为原料,用国产纤维素酶(酶活为13.94 FPU/100 mL)进行酶解试验,探索酶解最佳工艺条件。结果显示:底物固液比为0.47%(m/V),酶解pH值为4.70,酶解温度为50℃,酶解时间为72 h时酶解液还原糖含量最大,即糖得率最大,达59.41%。本研究也进行玉米秸秆经不同方法预处理后的酶解实验,结果显示其纤维素酶解效果顺序为:活性氧固体碱蒸煮浆>硫酸盐浆>机械粉碎>未预处理玉米秸秆。
The objective of this study was to explore technique of enzymatic hydrolysis of com stalk pulp （removal rate of lignin of 82.1%） from active oxygen cooking with a solid alkali with Chinese cellulase （enzyme activity： 13.94 FPU/10Oml）. The results showed that the optimum process conditions were the following： enzyme-substrate ratio of 0.47% （m/F）, pH value of 4.70, temperature of 50℃ and enzymatic reaction time of 72 h. The highest yield of sugar was 59.41% under the optimum process conditions. The paper also presented the different enzymolysis of corn stalk pretmated by different methods, and the order of the effect of enzymolysis was as follows： corn stalk pulp from active oxygen cooking with a solid base 〉 corn stalk pulp from alkaline cooking 〉〉 corn stalk pretreated by mechanical crushing 〉 raw corn stalk.
出处
《现代食品科技》
EI
CAS
北大核心
2012年第2期182-186,共5页
Modern Food Science and Technology
基金
国家重点基础研究发展计划(973计划)项目(2010CB732201)
华南理工大学轻工与食品实验教学示范中心建设项目资助
关键词
玉米秸秆浆
活性氧
固体碱
纤维素酶
酶解
corn stalk pulp
active oxygen
solid alkali
cellulase
enzymatic hydrolysis.
