中、高强度不同模式低氧训练对大鼠白细胞及其分类计数的影响

The Effect of Moderate and Heavy Intensity Hypoxic Training of Different Models on the Counting of White Blood Cells and Its Classification

目的:研究不同强度、不同方式低氧训练后白细胞及其分类计数的变化,探讨低氧环境和训练强度对机体免疫机能的影响.方法:200只大鼠按照氧环境(常氧、高住低练、高住高练)和训练强度(无训练、中强度、高强度)随机形成9种组合,5～6周中,高强度训练后于安静状态和定量运动后即刻取样,共分15小组.Advia 120Hematology System进行白细胞及其分类计数测定.结果:5～6周HiLo中,高强度训练后基础状态下表现为低WBC血症和中性粒显著上调,而且HiLo高训组在高强度定量运动后的WBC显著低于常氧和HiHi高训组;5～6周常氧和HiHi中,高强度训练对基础状态下白细胞及其分类计数无显著不良影响,HiHi中训组在中强度定量运动后甚至表现出白细胞免疫机能较常氧和HiLo中训组显著节省化现象;常氧、HiLo和HiHi环境下,高训组高强度定量运动后WBC变化均不显著,而中训组中强度定量运动后WBC均显著下降,提示中等强度大运动量运动后即刻可能存在白细胞免疫抑制现象.结论:HiLo训练对白细胞免疫机能极为不利,尤其是HiLo高强度训练;HiHi不仅没有表现出明显的不良症状,且中训组在定量运动后甚至表现出白细胞免疫机能节省化现象.

Objective： WBC and its classification of rats after hypoxic training of different intensity were studied to investigate the effects of different hypoxic environments and different training intensity on the immunity function of body.Methods：200 rats were randomly divided into 9 combinations according to the oxygen concentration（normal O2,living high and training low（HiLo）,living high and training high（HiHi））and the training intensity（no training,moderate intensity training（MT）and heavy intensity training（HT）） and 15 groups including quiet and exercise states.The period of training was 5 wks（MT） and 6 wks（HT）,respectively.WBC and its classification were measured by Advia 120 Hematology System.Results： 5 wks^6 wks of MT and HT of HiLo led to not only the up-regulation of Neut and the down-regulation of WBC in basic state,but also the WBC in HT of HiLo being significantly lower than that in HT of normal oxygen and HiHi after high intensity regular exercise.5 wks^6 wks of MT and HT in normal oxygen and HiHi did not show obvious adverse effects on the WBC and its classification,and the group of MT in HiHi even showed economizes on WBC immune function after the moderate intensity of regular exercise.The WBC decreased significantly after moderate intensity regular exercise in various oxygen environments,but it was not decreased after high intensity of regular exercise,suggesting that the phenomenon of the impression in immune system exist after moderate intensity and large amount of exercise immediately.Conclusion： HiLo is harmful to the WBC immune function,especially for the HT in HiLo.HiHi did not show obvious adverse effects on WBC,and the group of MT in HiHi even show economize on WBC immune function after the moderate intensity exercise.