链霉菌11371基因工程宿主菌的确定

Confirmation of Streptomyces 11371 Genetic Engineering Host Strain

为消除链霉菌11371内源性质粒对pIJ702的限制,以提高pIJ702转化率,确定11371基因工程宿主菌。采用种内接合转移的方法对链霉菌11371衍生菌U3和P2、P5、P7进行内源性质粒的消除。获得接合转移子U3-P7-6,具有转化外源质粒的能力,转化率为17～20个/100个菌,为链霉菌11371转化体系构建、克隆基因结构、功能鉴定和基因定位等研究提供生物材料。

In order to obliterate the restriction of the endogenous plasmid in Streptomyces ahygroscopicus subsp.wuzhouensis strain 11371 on pIJ702 to rise the conversion and confirm the genetic engineering host strain 11371,a method of intraspecific conjugal transferring was adopted to obliterate the endogenous plasmids in derived strains 11371 U3 and P2,P5,P7.The endogenous plasmid of derivative U3,P2,P5 and P7 of Streptomyces 11371 were obliterated by intraspecific conjugal transferring.The obtained conjugal transferant U3-P7-6 possessed the capability of converting exogenous plasmids,the conversion rate was 17~20/100 bacteria.This has provided bio-material for the study on the construction of converting system of Streptomyces 11371,gene structure cloning,function identification and genes localization.