施用胶质芽胞杆菌菌剂对黑麦草根际土壤脲酶、磷酸酶及过氧化氢酶活性的影响

Effects of Bacillus mucilaginosus Agents on the Activities of Urease,Phosphatase and Catalase in Ryegrass Rhizosphere Soil

土壤酶活性是反映土壤肥力最为重要的生物学指标之一。采用稀释涂布平板法研究了从玉米(K02)、草地早熟禾(K05)、披碱草(K09)、多年生黑麦草(K11)、匍匐翦股颖(K12)根际土壤中分离到的5株胶质芽胞杆菌菌株对黑麦草根际土壤脲酶、磷酸酶及过氧化氢酶活性的影响。结果表明:在苗期、中期、收获期各处理黑麦草根际土壤脲酶、磷酸酶和过氧化氢酶活性均高于对照(P<0.05)。总体来看,各处理黑麦草根际土壤脲酶和过氧化氢酶活性呈先增加后降低的变化趋势;土壤磷酸酶活性与对照(CK)相比除苗期处理K12外,其他处理均呈上升趋势,以处理K05磷酸酶活性最大。研究表明,施入胶质芽胞杆菌菌剂对黑麦草根际土壤脲酶、磷酸酶及过氧化氢酶活性有一定的积极作用,其结果可为生物钾肥的研制提供必要的数据。

Soil enzyme activity is one of the most important indexes that reflect soil fertility.This study was to investigate the effects of 5 Bacillus mucilaginosus（Bm） strains,isolated from rhizosphere of Zea mays（K02）,Poa pratenis（K05）,Elymusdahuricus（K09）,Lolium perenne（K11） and Agrostis palustris（K12）,respectively,on the activities of urease,phosphatase and catalase in ryegrass rhizosphere using a dilution coating flat method.The results showed that the urease and catalase activities demonstrated a tendency that increased firstly and then decreased,and alkaline phosphatase activity of all treatments showed the ascent trend compared with the control（CK）（P0.05）,except for the treatment K12 in its seeding stages,and the treatment K05 showed the most significant results than the others.This study indicated that Bm agents were beneficial to increase of urease,phosphatase and catalase activities in ryegrass rhizosphere.The results provide basis knowledge for future development of microbial potassium fertilizers.