大豆根瘤菌HH103菌株培养基的筛选与优化

Medium Screening and Optimization for Soy Bean Rhizobium (Rhizobium fredii) HH103

以快生型大豆根瘤菌HH103菌株为供试菌株,采用单因素碳氮源利用试验和正交设计试验,确定最佳培养基及其配方。结果表明:该菌株在YMA中生长良好,最佳碳源为蔗糖,最佳氮源为酵母膏,最佳培养基成分配方(g/L):蔗糖11,酵母膏0.9,K2HPO4 0.5,MnSO4 0.005,CaCl2 0.1,KH2PO4 0.5,MgSO4 0.2,KNO30.77,(NH4)2HPO4 0.33,FeCl3 0.005,pH 7.2。

Quick-growing soy bean rhizobium（Rhizobium fredii） HH103 was used as a tested strain,and adopted single factor nitrogen source utilization experiment and orthogonal design experiment to confirm the optimum medium and its formula.The results showed that the strain grew well on YMA,the optimum carbon source was sucrose,and the optimum nitrogen was yeast extract with the optimum medium formula as follows（in g/L）： sucrose 11,yeast extract 0.9,K2HPO4 0.5,MnSO4 0.005,CaCl2 0.1,KH2PO4 0.5,MgSO4 0.2,KNO3 0.77,（NH4）2HPO4 0.33,FeCl3 0.005,and pH 7.2.