不同发育时期荷瘤鼠脾组织结构的比较

Comparison of the structural change on different growth period tumor-bearing mice spleen

目的:探讨不同发育时期小鼠脾组织结构对荷瘤(S180)的反应。方法:取3个年龄段(20～25天龄、9～10周龄和12月龄)的昆明小鼠60只,每个年龄段又分为正常盐水对照组和肿瘤模型组。通过对小鼠脾指数、肿瘤指数,脾组织结构观察,体视学方法计量脾白髓相对面积百分比(AW)、单位面积脾小体的个数(NS)、单位面积脾树突状细胞个数、脾巨噬细胞截面直径等各项指标的测定分析,观察不同发育时期小鼠脾组织结构的不同特点以及对肿瘤的反应。结果:(1)随着年龄的增长,小鼠脾指数下降。在青年期和中老年期,小鼠荷瘤后脾脏显著增大,其差异有统计学意义。(2)肿瘤指数的年龄差别非常明显,各组差异有统计学意义。(3)除幼年荷瘤组的单位面积脾结构中脾小体个数较正常组增加外,各个不同发育时期荷瘤小鼠的AW与NS均比正常对照组下降。(4)青年期荷瘤小鼠脾巨噬细胞体积较正常组显著增大,而幼年期则成反向变化。结论:9～10周龄小鼠较其它组脾的各种结构对机体荷瘤更为敏感。

Objective： To explore the structural change of spleen on different growth period tumor - bearing mice. Meth- otis： 60 mice were divided into 6 groups： 20 -25-days normal saline control group ,20 - 25-days tumor control group,9 - 10-weeks normal saline control group, 9 -10-weeks tumor control group, 12-months normal saline control group, 12- months tumor control group. Evaluating of the experimental result included the tumor weight, tumor indexe, spleen weights, spleen index , relative areas of spleen white pulp （Aw ）, splenic nodule number （Ns）, spleen S100 ~ DC density and spleen macrophage diameter. Results： （1）Following aging , spleen index decreased, but, the spleen weights of tumor - bearing mice were increased in 9 - 10-weeks and 12-months . （2） The tumor index in 9 - 10-weeks tumor control group was significantly higher than the other groups （P 〈 0.01 ）. （3） AW and NS in tumor control group were descended than normal saline control group（ P 〈 0.01 ）, except the 20 - 25-days tumor control group. （4） The macrophage in 9 - 10 weeks tumor control group was larger than normal saline control group ,but it is reverse change in 20 ~ 25days mouse. Conclusion：The spleen of tumor - bearing mice in 9 - 10-weeks was more sensitive than others.